Anti-Acetyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM172; H3K27ac, Histone H3 (K27 acetylation)

$155.00$370.00

Rabbit monoclonal to Acetylated Histone H3 Lysine 27 (K27ac), H3K27ac, Histone H3 (K27 acetylation)

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Product Details
Product NameAnti-Acetyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody [RM172]
Product DescriptionRabbit monoclonal to Acetylated Histone H3 Lysine 27 (K27ac), H3K27ac, Histone H3 (K27 acetylation)
Catalog No.31-1056-00
Clone NameRM172
Product Data SheetAnti-Acetyl-Histone H3 Lys27 Rabbit Monoclonal Antibody Data Sheet RM172
SpecificityRM172 reacts to Histone H3 acetylated at Lysine 27 (K27ac). No cross reactivity with other acetylated Lysines in histone H3.
ImmunogenAn acetyl-peptide corresponding to Acetyl-Histone H3 (Lys27).
ApplicationWestern Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Chromatin IP (ChIP)
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.5 ug/mL - 2 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.

Keywords
References for Acetyl-Histone H3 (Lys27) antibody [RM172]Li Y et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. (2016) 113(50):14402-14407. ChIP; Human.Read more (PubMed: 27911794)

Bulut-Karslioglu A et al. The Transcriptionally Permissive Chromatin State of Embryonic Stem Cells Is Acutely Tuned to Translational Output. Cell Stem Cell. (2018) 10.1016/j.stem.2018.02.004. WB; Human. Read more (PubMed: 29499153)

Lu P et al. Genome encode analyses reveal the basis of convergent evolution of fleshy fruit ripening. Nat Plants. (2018) 10.1038/s41477-018-0249-z. ChIP; Plant. Read more (PubMed: 30250279)
Product Images
RM172

RM172 specifically reacts to Histone H3
acetylated at Lysine 27 (K27ac). No cross reactivity
with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac),
Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23
(K23ac), Lysine 36 (K36ac), Lysine 56 (K56ac),
Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.

Western

Western Blot of acid extracts from HeLa cells
untreated (-) or treated with sodium butyrate (+),
using RM172 at 1 ug/mL, showed a band of
histone H3 acetylated at Lysine 27 in treated HeLa.

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H3 (Lys27)
Rabbit mAb RM172 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).


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Size Format

25ug – $155, 100ug – $370

References

Li Y et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. (2016) 113(50):14402-14407. ChIP; Human.Read more (PubMed: 27911794)

Bulut-Karslioglu A et al. The Transcriptionally Permissive Chromatin State of Embryonic Stem Cells Is Acutely Tuned to Translational Output. Cell Stem Cell. (2018) 10.1016/j.stem.2018.02.004. WB; Human. Read more (PubMed: 29499153)

Lu P et al. Genome encode analyses reveal the basis of convergent evolution of fleshy fruit ripening. Nat Plants. (2018) 10.1038/s41477-018-0249-z. ChIP; Plant. Read more (PubMed: 30250279)