Anti-Acetyl-Histone H4 (Lys12) Rabbit Monoclonal Antibody, Clone RM202; H4K12ac, Histone H4 (K12 acetylation)


Rabbit monoclonal to Acetyl-Histone H4 (Lys12), H4K12ac, Histone H4 (K12 acetylation)

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Product Details
Product NameAnti-Acetyl-Histone H4 (Lys12) Rabbit Monoclonal Antibody [RM202]
Product DescriptionRabbit monoclonal to Acetylated Histone H4 Lysine 12 (K12ac), H4K12ac, Histone H4 (K12 acetylation)
Catalog No.31-1088-00
Clone NameRM202
Product Data SheetAnti-Acetyl-Histone H4 Lys12 Rabbit Monoclonal Antibody Data Sheet RM202
SpecificityRM202 reacts to Histone H4 acetylated at Lysine 12 (K12ac). No cross reactivity with other acetylated Lysines in Histone H4.
ImmunogenAn acetyl-peptide corresponding to Acetyl-Histone H4 (Lys12).
ApplicationWestern Blot (WB)
Immunocytochemistry (ICC)
Species ReactivityHuman
Intended UseResearch Use Only
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.5 ug/mL - 1 ug/mL;
Multiplex: 0.5 ug/mL – 2 ug/mL;
Immunocytochemistry: 0.5 ug/mL- 2 ug/mL.

Product Images

RM202 specifically reacts to Histone H4 acetylated
at Lysine 12 (K12ac). No cross reactivity with unmodified
Lysine 16 (K16 ctrl), acetylated Lysine 5 (K5ac), Lysine
8 (K8ac), Lysine 16 (K16ac), Lysine 20 (K20ac),
Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.


Western Blot of acid extracts from: (1) HeLa cells treated
with sodium butyrate; (2) HeLa cells untreated. Using
RM202 at 0.5 ug/mL, showed a band of Histone H4
acetylated at Lysine 12 in HeLa cells.


Immunocytochemical staining of HeLa cells treated
with sodium butyrate, using anti-Acetyl-Histone
H4 (Lys12) Rabbit Monoclonal Antibody (clone
RM202) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).

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25ug – $155, 100ug – $360

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