Anti-Dimethyl-Histone H3 (Lys23) Rabbit Monoclonal Antibody, Clone RM171; H3K23me2, Histone H3 (dimethyl K23)

$155.00$360.00

Rabbit monoclonal to Dimethylated Histone H3 Lysine 23, H3K23me2, Histone H3 (dimethyl K23)

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Product Details
Product NameAnti-Dimethyl-Histone H3 (Lys23) Rabbit Monoclonal Antibody [RM171]
Product DescriptionRabbit monoclonal to Dimethylated Histone H3 Lysine 23, H3K23me2, Histone H3 (dimethyl K23)
Catalog No.31-1077-00
Clone NameRM171
Product Data SheetAnti-Dimethyl-Histone H3 (Lys23) Rabbit Monoclonal Antibody Data Sheet RM171
SpecificityRM171 reacts to Histone H3 dimethylated at Lysine 23 (K23me2). No cross reactivity with nonmodified Lysine 23, monomethylated Lysine 23 (K23me1), or other methylations in histone H3.
ImmunogenA dimethyl-peptide corresponding to dimethyl-Histone H3 (Lys23).
ApplicationWestern Blot (WB)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.1 ug/mL - 0.5 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.

Keywords
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Product Images
A

A Peptide dotblot shows RM171 only reacts to Histone.
H3 dimethyl-Lysine 23 (K23me2). No cross reactivity.
with nonmodified Lysine 23 (K23Ctrl), monomethylated.
Lysine 23 (K23me1), or dimethylated Lysine 4 (K4me2).

RM171

RM171 specifically reacts to Histone H3 dimethylated
at Lysine 23 (K23me2). No cross reactivity with other
methylated lysines in Histone H3.

Western

Western Blot of recombinant histone H3.3 (1)
and acid extracts of HeLa cells (2), using RM171
at 0.5 ug/mL, showed a band of histone H3
dimethylated at Lysine 23 (K23me2) in HeLa cells.


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