RM141 specifically reacts to Histone H3 dimethylated Western Blot of recombinant histone H3.3 (1)
Product Details
Product Name Anti-Dimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody, Clone RM141
Product Description Rabbit monoclonal to Dimethylated Histone H3 Lysine 36, H3K36me2, Histone H3 (dimethyl K36)
Catalog No. 31-1047-00
Clone Name RM141
Product Data Sheet Anti-Dimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody Data Sheet RM141
Specificity RM141 reacts to Histone H3 dimethylated at Lysine 36 (K36me2). No cross reactivity with monomethylated Lysine 36 (K36me1), trimethylated Lysine 36 (K36me3), or other methylations in histone H3.
Immunogen A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys36).
Application Western Blot (WB)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 µg/mL – 2 µg/mL;
ELISA: 0.2 µg/mL - 1 µg/mL;
Multiplex: 0.1 µg/mL – 0.5 µg/mL.
Keywords
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Product Images
at Lysine 36 (K36me2). No cross reactivity with non
-modified Lysine 36 (K36 Ctrl), monomethylated
Lysine 36 (K36me1), trimethylated Lysine 36
(K36me3), or other methylations in Histone H3.
and acid extracts of HeLa cells (2), using RM141
at 0.5 µg/mL, showed a band of histone H3
dimethylated at Lysine 36 (K36me2) in HeLa cells.
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