Anti-Histone H3.3 G34V Rabbit Monoclonal Antibody, Clone RM307; Histone H3.3 G34V Mutant (50ug)

$375.00

Rabbit monoclonal to Histone H3.3 G34V; Histone H3.3 G34V Mutant

Product Details
Product NameAnti-Histone H3.3 G34V Rabbit Monoclonal Antibody [RM307]
Product DescriptionRabbit monoclonal to Histone H3.3 G34V; Histone H3.3 G34V Mutant
Catalog No.31-1193-00
Clone NameRM307
Product Data SheetAnti-Histone H3.3 G34V Mutant Rabbit Monoclonal Antibody Data Sheet RM307
SpecificityRM307 reacts to the Histone H3.3 G34V mutant. No cross reactivity with wild type Histone H3.3 .
ImmunogenA peptide corresponding to Histone H3.3 G34V mutant
ApplicationWestern Blot
ELISA
Immunohistochemistry
Immunocytochemistry
Species ReactivityAll
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size50 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.02 ug/mL – 0.5 ug/mL;
ELISA: 0.05 ug/mL - 0.5 ug/mL;
Immunohistochemistry (IHC): 0.01 – 0.5 ug/mL;
Immunocytochemistry (ICC): 0.01 – 0.5 ug/mL.
References for Histone H3.3 G34V Mutant antibody [RM307]Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742)
.
Product Images
Western

Western Blot analysis of cell lysates prepared from
293T, transfected with a DNA construct encoding
wild type or G34V mutant proteins of Histone H3.3,
using anti-Histone H3.3 G34V rabbit monoclonal
antibody (clone RM307) at 0.02ug/mL.

Immunohistochemical

Immunohistochemical staining of formalin fixed and
paraffin embedded 293T cells transfected with a
DNA construct encoding Histone H3.3 wild type
or G34V mutant, stained with anti-Histone H3.3
G34V rabbit monoclonal antibody (clone RM307)
at 0.01ug/mL.


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References

Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742)