Anti-Human IgG3 Rabbit Monoclonal Antibody, Clone RM119

$265.00

Rabbit monoclonal to Human IgG3

SKU: 31-1021-00. Categories: , , . Tags: , .

Product Details
Product NameAnti-Human IgG3 Rabbit Monoclonal Antibody [RM119]
Product DescriptionRabbit monoclonal to Human IgG3
Catalog No.31-1021-00
Clone NameRM119
Product Data SheetAnti-Human IgG3 Rabbit Monoclonal Antibody Data Sheet RM119
SpecificityRM119 reacts to the heavy chain of human IgG3. No cross reactivity with human IgG1, IgG2, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
ImmunogenHuman IgG3
ApplicationImmunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityHuman
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Keywords
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Product Images

Immunohistochemistry of Human Lymphoid Tissue using
Anti-Human IgG3 antibody RM119.

Sandwich

Sandwich ELISA using RM119 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an HRP conjugated streptavidin.

Sandwich

Sandwich ELISA using RM119 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an HRP conjugated streptavidin.

ELISA

ELISA of human immunoglobulins shows RM119
reacts only to human IgG3. No cross reactivity
with Human IgG1, IgG2, IgG4, IgE, IgD, IgA,
mouse IgG, rat IgG, or goat IgG. The plate was
coated with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL, or 10 ng/mL of RM119 was used
as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.

A

A titer ELISA using RM119. The plate was coated
with different amounts of human IgG3. A serial dilution
of RM124 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.

Cross-activity data of RevMAb anti-human IgG antibodies against
monkey IgG. ELISA plate was coated with 0.1ug/well (2ug/mL,
50uL/well) of Rhesus monkey IgG (protein A purified from serum).
A serial dilution of anti-human IgG (RM116), anti-human IgG1
(RM117), anti-human IgG2 (RM118), anti-human IgG3 RM119),
(anti-human IgG4 (RM120), anti-human kappa (RM126), and
anti-human lambda (RM127) was used as the primary antibody.
An alkaline phosphatase conjugated anti-rabbit IgG as the secondary
antibody.


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