Anti-Human IgG3 Rabbit Monoclonal Antibody, Clone RM119

$265.00

Rabbit monoclonal to Human IgG3

SKU: 31-1021-00. Categories: , , . Tags: , .

Product Details
Product NameAnti-Human IgG3 Rabbit Monoclonal Antibody [RM119]
Product DescriptionRabbit monoclonal to Human IgG3
Catalog No.31-1021-00
Clone NameRM119
Product Data SheetAnti-Human IgG3 Rabbit Monoclonal Antibody Data Sheet RM119
SpecificityRM119 reacts to the heavy chain of human IgG3, RM119 does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG4), and shows no cross reactivity IgM, IgA, IgD, IgE. RM119 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
ImmunogenHuman IgG3
ApplicationImmunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityHuman
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Keywords
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Product Images
Detection

Detection of human IgG3 in monkey serum.
Sandwich ELISA using RM119 as the capture
antibody, and biotinylated anti-human light
chains ( κ+ λ) antibody RM129 as the detection
antibody, followed by a HRP conjugated streptavidin.

Sandwich

Sandwich ELISA using RM119 as the capture antibody
(100ng/well), and biotinylated anti-human light chains ( κ+ λ)
antibody RM129 as the detection antibody,
followed
by an HRP conjugated streptavidin.

ELISA

ELISA showing RM119 reacts only to human IgG3,
and not to any other IgG subclasses (IgG1, IgG2,
or IgG4), and no cross reactivity to IgM, IgA, IgD,
IgE, mouse IgG, rat IgG, or goat IgG.

A

A titer ELISA using RM119. The plate was coated
with different amounts of human IgG3. A serial dilution
of RM119 was used as the primary antibody, followed
by an alkaline phosphatase conjugated anti-rabbit IgG
as the secondary antibody.

Immunohistochemical staining of formalin fixed
and paraffin embedded human lymphoid tissue
section using anti-human IgG3 rabbit monoclonal
antibody (Clone RM119).

ELISA

ELISA showing RM119 does not react to monkey
IgG. The plate was coated with Rhesus monkey IgG.
A serial dilution of RM119 and a monkey IgG binding
antibody (positive control) was used as the detection
antibody.


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