Anti-Monomethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody, Clone RM147; H3K79me1, Histone H3 (monomethyl K79)

$155.00$369.00

Rabbit monoclonal to Monomethylated Histone H3 Lysine 79, H3K79me1, Histone H3 (monomethyl K79)

Clear selection

Product Details
Product NameAnti-Monomethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody [RM147]
Product DescriptionRabbit monoclonal to Monomethylated Histone H3 Lysine 79, H3K79me1, Histone H3 (monomethyl K79)
Catalog No.31-1049-00
Clone NameRM147
Product Data SheetAnti-Monomethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody Data Sheet RM147
SpecificityRM147 reacts to Histone H3 monomethylated at Lysine 79 (K79me1). No cross reactivity with dimethylated Lysine 79 (K79me2) or trimethylated Lysine 79 (K79me3), or other methylations in histone H3
ImmunogenA monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys79).
ApplicationWestern Blot (WB)
Chromatin IP (ChIP)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.2 ug/mL – 1 ug/mL;
ChIP: 2 ug/mL- 10 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.

Keywords
.
Product Images
RM147

RM147 specifically reacts to Histone H3 monomethylated
at Lysine 79 (K79me1). No cross reactivity with dimethylated
Lysine 79 (K79me2), trimethylated Lysine 79 (K79me3), or
other methylations in histone H3.

Western

Western Blot of recombinant histone H3.3 (1) and
acid extracts of HeLa cells (2), using RM147 at 0.5
ug/mL, showed a band of histone H3 mono-
methylated at Lysine 79 (K79me1) in HeLa cells.

ChIP

ChIP performed on HeLa cells using H3K79me1 antibody
(RM147, 5ug). Real-time PCR was performed using primers
specific to the gene indicated.


Related Products

Size Format

25ug – $155, 100ug – $369

Related Products