Anti-Mouse IgG3 Rabbit Monoclonal Antibody, Clone RM218

$290.00

Rabbit monoclonal to Mouse IgG3

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SKU: 31-1094-00. Categories: , , . Tags: , .

Product Details
Product NameAnti-Mouse IgG3 Rabbit Monoclonal Antibody [RM218]
Product DescriptionRabbit monoclonal to Mouse IgG3
Catalog No.31-1094-00
Clone NameRM218
Product Data SheetAnti-Mouse IgG3 Rabbit Monoclonal Antibody Data Sheet RM218
SpecificityRM218 reacts to the Fab region of mouse IgG3. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG.
ImmunogenMouse IgG3
ApplicationWestern Blot (WB)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityMouse
ConjugateNone
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 0.005 ug/mL-0.2 ug/mL;
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;
Western Blot (WB): 0.1 ug/mL-0.5 ug/mL.
Keywords
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Product Images

ELISA of mouse immunoglobulins shows RM218
reacts to both mouse IgG3κ and IgG3λ; No cross
reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA,
IgE, human IgG, or rat IgG. The plate was coated
with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL, or 10 ng/mL of RM218 was
used as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.

A titer ELISA of mouse IgG3. The plate was coated
with different amounts of mouse IgG3. A serial
dilution of RM218 was used as the primary antibody.
An alkaline phosphatase conjugated anti-rabbit IgG
as the secondary antibody.


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