Western blot of nonreduced(-) and reduced(+) ELISA of mouse immunoglobulins shows RM103 A titer ELISA of mouse IgG1κ. The plate was coated
Product Name Anti-Mouse Immunoglobulin Kappa Light Chain Rabbit Monoclonal Antibody [RM103]
Product Description Rabbit monoclonal to Mouse Ig Kappa Light Chain
Catalog No. 31-1007-00
Clone Name RM103
Product Data Sheet Anti-Mouse Ig Kappa Rabbit Monoclonal Antibody Data Sheet RM103
Specificity This antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG.
The Fc region of RM103 has been engineered to eliminate Fc receptor binding.
Immunogen Mouse IgG
Application Western Blot (WB) (nonreduced)
Flow Cytometry (FC)
Species Reactivity Mouse
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.005ug/mL-0.2ug/mL;
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL;
Western Blot (WB): 0.1ug/mL-0.5ug/mL.
Keywords . Product Images
mouse IgG1κ and IgG1λ (20ng/lane), using 0.2ug/mL
of RevMAb clone RM103. This antibody reacts to
nonreduced IgG1κ (~150 kDa), and slightly reacts
to reduced κ light chain (~25 kDa).
reacts to the kappa light chain of mouse immuno-
globulins. No cross reactivity with the lamda light
chain, human IgG (κ+λ), rat IgG (κ+λ), or goat IgG
(κ+λ). The plate was coated with 50 ng/well of
different immunoglobulins. 200 ng/mL, 50 ng/mL,
or 10 ng/mL of RM103 was used as the primary
antibody. An alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.
with different amounts of mouse IgG1κ. A serial
dilution of RM103 was used as the primary antibody.
An alkaline phosphatase conjugated anti-rabbit
IgG as the secondary antibody.
Western blot of nonreduced(-) and reduced(+)
ELISA of mouse immunoglobulins shows RM103
A titer ELISA of mouse IgG1κ. The plate was coated