Anti-Phosphotyrosine Rabbit Monoclonal Antibody, Clone RM111

$370.00

Rabbit monoclonal to Phosphotyrosine

Product Details
Product NameAnti-Phosphotyrosine Rabbit Monoclonal Antibody [RM111]
Product DescriptionRabbit monoclonal to Phosphotyrosine
Catalog No.32-1011-00
Clone NameRM111
Product Data SheetAnti-Phosphotyrosine Rabbit Monoclonal Antibody Data Sheet RM111
SpecificityRM111 reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine
ImmunogenPhosphotyrosine-BSA conjugate
ApplicationWestern Blot (WB)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityAll Species
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot (WB): 1:1000 -1:5000 dilution;
Immunoprecipitation (IP): 1:200-1:1000 dilution;
Chromatin IP (ChIP): 1:200-1:1000 dilution;
Immunocytochemistry (ICC): 1:200-1:500 dilution;
Immunohistochemistry (IHC): 1:200-1:500 dilution.
Keywords
References for Phosphotyrosine Rabbit Monoclonal Antibody [RM111]Sun Y et al. Neuregulin-1/ErbB4 Signaling Regulates Visual Cortical Plasticity Neuron (2016) 10.1016/j.neuron.2016.08.033. IHC; Mouse. Read more (PubMed: 27641496)

Product Images
Immunocytochemistry

Immunocytochemistry of serum-starved A431
cells nontreated or treated with EGF, using
RevMAb Clone RM111 at 1/500 dilution (foll-
owed by a PE conjugated secondary antibody,
red) and DAPI (blue).

Western

Western blot of serum-starved A431 cells non-
treated or treated with EGF, using RevMAb
Clone RM111 at 1/5000 dilution. The blot was
exposed to the film at different time points.

Immunoprecipitation

Immunoprecipitation of EGF-treated A431 cells
by RevMAb Cone RM111 at 1/1000 dilution, was
blotted with an anti-EGFR rabbit monoclonal antibody.
(1) Whole lysate control; (2) IP by rabbit IgG control;
(3) IP by Clone RM111.

Luminex

Luminex analysis of Clone RM111’s reaction
against phosphorylated or nonphosphorylated
Serine, Threonine, and Tyrosine. RevMAb Clone
RM111 reacts only to phosphorylated Tyrosine.


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References

Sun Y et al. Neuregulin-1/ErbB4 Signaling Regulates Visual Cortical Plasticity Neuron (2016) 10.1016/j.neuron.2016.08.033. IHC; Mouse. Read more (PubMed: 27641496)