Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM137; H3K4me3, Histone H3 (trimethyl K4)

$155.00$365.00

Rabbit monoclonal to Trimethylated Histone H3 Lysine 4, H3K4me3, Histone H3 (trimethyl K4)

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Product Details
Product NameAnti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [RM137]
Product DescriptionRabbit monoclonal to Trimethylated Histone H3 Lysine 4, H3K4me3, Histone H3 (trimethyl K4)
Catalog No.31-1039-00
Clone NameRM137
Product Data SheetAnti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM137
SpecificityRM137 reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with monomethylated Lysine 4 (K4me1), dimethylated Lysine 4 (K4me3), or other methylations in histone H3.
ImmunogenA trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys4).
ApplicationWestern Blot
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.2 ug/mL – 1 ug/mL;
ELISA: 0.1 ug/mL – 0.5 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.

References for Trimethyl-Histone H3 (Lys4) antibody [RM137]Lu P et al. Genome encode analyses reveal the basis of convergent evolution of fleshy fruit ripening. Nat Plants. (2018) 10.1038/s41477-018-0249-z. ChIP; Plant. Read more (PubMed: 30250279)
.
Product Images
A

A Peptide dotblot shows RM137 reacts only to
Histone H3 trimethyl-Lysine 4 (K4me3). No cross
reactivity with nonmodified Lysine 4 (H3N1-19),
monomethylated Lysine 4 (K4me1) or dimethylated
Lysine 4 (K4me2).

RM137

RM137 specifically reacts to Histone H3 trimethylated
at Lysine 4 (K4me3). No cross reactivity with other
methylated lysines in Histone H3.

Western

Western Blot of recombinant histone H3.3 (1) and
acid extracts of HeLa cells (2), using RM137 at
0.5 ug/mL, showed a band of histone H3 tri-
methylated at Lysine 4 (K4me3) in HeLa cells.


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Size Format

25ug – $155, 100ug – $365

References

Lu P et al. Genome encode analyses reveal the basis of convergent evolution of fleshy fruit ripening. Nat Plants. (2018) 10.1038/s41477-018-0249-z. ChIP; Plant. Read more (PubMed: 30250279)