Immunohistochemical staining of formalin fixed and paraffin embedded Immunohistochemical staining of formalin fixed and paraffin embedded Immunohistochemical staining of formalin fixed and paraffin embedded Western Blot analysis of A375 cell (BRAF V600E) and HEK293 cell (WT) Immunohistochemical staining of formalin fixed and Immunohistochemical staining of formalin fixed and
Product Details
Product Name BRAF V600E Rabbit Monoclonal Antibody, Clone RM8
Product Description Rabbit monoclonal to BRAF V600E Mutant, BRAF(V600E), BRAF-V600E
Catalog No. 31-1042-00;
31-1042-00-S;
31-1042-02
Clone Name RM8
Product Data Sheet BRAF V600E Rabbit Monoclonal Antibody Data Sheet
Biotinylated BRAF V600E Rabbit Monoclonal Antibody Data Sheet
Specificity RM8 reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF.
Immunogen A peptide corresponding to BRAF V600E mutant
Application Immunohistochemistry
Immunocytochemistry
Western Blot
ELISA
Flow Cytometry
Protocols BRAF V600E IHC Protocol
Species Reactivity Human
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 µg;
50 µg;
Biotinylated 50 µg
Concentration 1 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Immunohistochemistry (IHC): 0.5 µg/mL – 5 µg/mL;
Immunocytochemistry (ICC): 0.5 µg/mL – 5 µg/mL;
Western Blot (WB): 0.5 µg/mL – 2 µg/mL;
ELISA: 0.5 µg/mL – 2 µg/mL;
References for BRAF V600E Mutant antibody [RM8] Labib M et al. Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting. Nat Biomed Eng. (2020), 10.1038/s41551-020-0590-1. FC; Human. Read more (PubMed: 32719513)
Crespo-Rodriguez E et al. Combining BRAF inhibition with oncolytic herpes simplex virus enhances the immune-mediated antitumor therapy of BRAF-mutant thyroid cancer. J Immunother Cancer. (2020), 10.1136/jitc-2020-000698. WB; Human. Read more (PubMed: 32759235)
Martin AM et al. PD-L1 Expression in Pediatric Low-Grade Gliomas Is Independent of BRAF V600E Mutational Status. J Neuropathol Exp Neurol. (2020), 10.1093/jnen/nlz119. WB; Human. Read more (PubMed: 31819973)
Shirsat PM et al. Low frequency of BRAF V600E immunoexpression in mandibular ameloblastomas: An institutional study. J Oral Maxillofac Pathol. (2018), 10.4103/jomfp.JOMFP_174_17. IHC; Human. Read more (PubMed: 30651680)
Usman H et al. Result Analysis of BRAF V600E Gene Mutation Using Molecular and Immunohistochemistry Detection in Acral Malignant Melanoma. Asian Pacific Journal of Cancer Care. (2018), 10.31557/APJCC.2018.3.3.43. IHC; Human. Read more (WAOCP)
Hansen C et al. Nanoswitch-linked immunosorbent assay (NLISA) for fast, sensitive, and specific protein detection. Proc Natl Acad Sci USA. (2017), 10.1073/pnas.1708148114. ELISA, WB; Human. Read more (PubMed: 28893984)
Krishnamurthy A et al. Clinical utility of immunohistochemistry using the novel anti-BRAF V600E antibody (clone RM8) for detection of the BRAF V600E mutant protein in papillary thyroid cancers. Int J Mol ImmunoOncol. (2018) 10.18203/issn.2456-3994.IntJMolImmunoOncol20180471. IHC-P; Human. Read more (IJMIO: ISSN: 2456-3994)
Gouravan S et al. Preclinical Evaluation of Vemurafenib as Therapy for BRAFV600E Mutated Sarcomas. Int J Mol Sci. (2018) 10.3390/ijms19040969. WB; Human. Read more (PubMed: 29570692)
Yang H et al. Activated MEK cooperates with Cdkn2a and Pten loss to promote the development and maintenance of melanoma. Oncogene (2017) 10.1038/onc.2016.526. WB; Human. Read more (PubMed: 28263969)
ZM Shaban et al. BRAFV600E Mutation in Gliomas in Iraqi Patients Immunohistochemical Study. ijcocr (2017) 10.11648/j.ijcocr.20170206.11. IHC; Human. Read More
Product Images
melanoma tissue sections using anti-BRAF V600E rabbit monoclonal antibody, Clone RM8,
at 2 µg/mL.
Thyroid Carcinoma tissue using anti-BRAF V600E rabbit monoclonal antibody, Clone RM8,
at 0.5 µg/mL. Allele specific PCR validated to be Negative for BRAF V600E.
Thyroid Carcinoma tissue using anti-BRAF V600E rabbit monoclonal antibody, Clone RM8,
at 0.5 µg/mL. Allele specific PCR validated to be Positive for BRAF V600E.
lysates using anti-BRAF V600E rabbit monoclonal antibody, Clone RM8,
at 2 µg/mL.
paraffin embedded human cell line sections.
WiDr Cells (400X, Positive)
paraffin embedded human cell line sections.
SW40 Cells (400X, Negative)
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