BRAF V600E Rabbit Monoclonal Antibody, Clone RM8; BRAF(V600E)

$199.00$499.00

Rabbit monoclonal to BRAF V600E Mutant, BRAF(V600E), BRAF-V600E;
25 ug Sample Size, 100 ug Full Size
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Product Details
Product NameBRAF V600E Rabbit Monoclonal Antibody [RM8]
Product DescriptionRabbit monoclonal to BRAF V600E Mutant, BRAF(V600E), BRAF-V600E
Catalog No.31-1042-00
Clone NameRM8
Product Data SheetBRAF V600E Rabbit Monoclonal Antibody Data Sheet
SpecificityRM8 reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF.
ImmunogenA peptide corresponding to BRAF V600E mutant
ApplicationImmunohistochemistry
Immunocytochemistry
Western Blot
Species ReactivityHuman
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration 1 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageImmunohistochemistry (IHC): 0.5 – 5 ug/mL;
Immunocytochemistry (ICC): 2 – 10 ug/mL;
Western Blot (WB): 0.5 – 2 ug/mL
References for BRAF V600E Mutant antibody [RM8]Sun HL et al. ERK Activation Globally Downregulates miRNAs through Phosphorylating Exportin-5. Cancer Cell 10.1016/j.ccell.2016.10.001. WB; Human. Read more (PubMed: 27846390)

Bajaj J et al. CD98-Mediated Adhesive Signaling Enables the Establishment and Propagation of Acute Myelogenous Leukemia. Cancer Cell 10.1016/j.ccell.2016.10.003. WB; Human. Read more (PubMed: 27908736)

Yang Het al. Activated MEK cooperates with Cdkn2a and Pten loss to promote the development and maintenance of melanoma. Oncogene 10.1038/onc.2016.526. WB; Human. Read more (PubMed: 28263969)

ProtocolsBRAF V600E IHC Protocol
Product Images
Immunohistochemical

Immunohistochemical staining of formalin fixed and
paraffin embedded melanoma tissue sections.

Immunohistochemical

Immunohistochemical staining of formalin fixed and
paraffin embedded Thyroid Carcinoma tissue. Allele
specific PCR validated to be Negative for BRAF V600E.

Immunohistochemical

Immunohistochemical staining of formalin fixed and
paraffin embedded Thyroid Carcinoma tissue. Allele
specific PCR validated to be Positive for BRAF V600E.

Western

Western Blot analysis of cell lysates prepared from cell
lines expressing endogenous mutant (V600E) BRAF or
wild type (WT) protein.

Immunohistochemical

Immunohistochemical staining of formalin fixed and
paraffin embedded human cell line sections.


WiDr Cells (400X, Positive)

Immunohistochemical

Immunohistochemical staining of formalin fixed and
paraffin embedded human cell line sections.



SW40 Cells (400X, Negative)


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Size Format

25ug – $199, 100ug – $499

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