Detection of 5-Carboxylcytosine (5-caC), in linear denatured DNA (426 bp).
Product Details
Product Name 5-Carboxylcytosine (5-caC) DNA Matched Antibody Pair [RM462/RM476]
Product Description This antibody pair detects 5-Carboxylcytosine (5-caC) in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-mC, 5-hmC, or 5-fC) in DNA. This product includes capture and detection antibodies for Sandwich ELISA assays.
Catalog No. 31-1354-MK
Clone Name RM462 Anti-5-Carboxylcytosine (5-caC) Rabbit Monoclonal Antibody; BSA Free
RM476 Biotin Anti-Adenine and Guanine Rabbit Monoclonal Antibody
Product Data Sheet 5-Carboxylcytosine (5-caC) DNA Matched Antibody Pair Datasheet
Specificity This antibody pair detects 5-Carboxylcytosine (5-caC) in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-mC, 5-hmC, or 5-fC) in DNA.
Application Sandwich ELISA, or other Sandwich Assays
Species Reactivity Human
Conjugate RM476; Biotin
Intended Use Research Use Only
Related Products Anti-5-Carboxylcytosine (5-caC) Rabbit Monoclonal Antibody, Clone RM462
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Components 1) Capture Antibody: Anti-5-Carboxylcytosine (5-caC) Rabbit Monoclonal Antibody, Clone RM462, 50 μg at 1.0 mg/mL in 50% Glycerol/PBS with 0.09% sodium azide, without BSA or other carrier proteins.
2) Detection Antibody: Biotin Anti-Adenine and Guanine Rabbit Monoclonal Antibody, Clone RM476, 25 μg at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Usage ELISA: Capture Antibody 50 ng/well – 200 ng/well;
Detection Antibody 0.1 μg/mL – 0.5 μg/mL
Keywords
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Product Images
Sandwich ELISA using anti-Carboxylcytosine (5-caC) (clone RM462) as the
capture antibody (50 ng/well at 1 μg/mL), and biotinylated anti-Adenine and
Guanine (Clone RM476) as the detection antibody (0.2 μg/mL), followed by
an AP conjugated streptavidin.
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