Dot blot of DNA using anti-5-fC Rabbit Monoclonal Antibody, Clone RM477. ELISA of DNA using anti-5-fC Rabbit Monoclonal Antibody, Clone RM477 (1 µg/mL).
Product Details
Product Name Anti-5-Formylcytosines (5-fC) Rabbit Monoclonal Antibody, Clone RM477
Product Description Rabbit monoclonal to 5-Formylcytosines (5-fC) Rabbit Monoclonal Antibody, 5'-fC
Catalog No. 31-1369-00
Clone Name RM477
Product Data Sheet Anti-5-Formylcytosines (5-fC) Rabbit Monoclonal Antibody Data Sheet RM477
Specificity RM477 reacts to 5-Formylcytosines (5-fC) in DNA. No cross reactivity with non-modified cytosine and other modified cytosines in DNA.
Immunogen KLH-conjugated 5-Formylcytosines.
Application Dot Blot
ELISA
Species Reactivity All
Conjugate None
Intended Use Research Use Only
Related Product 5-Formylcytosines (5-fC) DNA Matched Antibody Pair
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt.
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 50 μg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Dot Blot: 0.5 μg/mL - 2 μg/mL
ELISA: 0.5 μg/mL - 2 μg/mL
Keywords
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Product Images
The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of linear denatured
DNA (426 bp) containing either normal cytosines (unmodified), 5-methylcytosines
(5-mC), 5-hydroxymethylcytosines (5-hmC), 5-carboxylcytosines (5-caC), or
5-formylcytosines (5-fC).
The plate was coated with different concentrations of linear denatured DNA (426 bp)
containing either normal cytosines (unmodified), 5-methylcytosines (5-mC),
5-hydroxymethylcytosines (5-hmC), 5-carboxylcytosines (5-caC), or
5-formylcytosines (5-fC).
Related Products
DNA Methylation
5-Formylcytosines (5-fC) DNA Matched Antibody Pair [RM477/RM476]
Matched Antibody Pair Kits
5-Carboxylcytosine (5-caC) DNA Matched Antibody Pair [RM462/RM476]
DNA Methylation
5-Methylcytosine (5-mC) DNA Matched Antibody Pair [RM231/RM476]
DNA Methylation
Anti-5-hydroxymethylcytosine (5-hmC) Rabbit Monoclonal Antibody, Clone RM236; 5′-hmC