RM222 specifically reacts to Histone H2A.Z acetylated Western Blot of acid extracts from HeLa cells Immunocytochemical staining of HeLa cells treated
Product Details
Product Name Anti-Acetyl Histone H2A.Z (Lys7) Rabbit Monoclonal Antibody, Clone RM222
Product Description Rabbit monoclonal to Acetyl Histone H2.AZ (Lys7)
Catalog No. 31-1102-00
Clone Name RM222
Product Data Sheet Anti-Acetyl-Histone H2.AZ (Lys7) Rabbit Monoclonal Antibody Data Sheet RM222
Specificity RM222 reacts to Histone H2A.Z acetylated at Lysine 7 (K7ac). No cross reactivity with non-modified Lysine 7 or other acetylated Lysines in histone H2A.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H2A.Z (Lys7).
Application Western Blot (WB)
ELISA
Multiplex
Immunocytochemistry (ICC)
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 µg/mL – 2 µg/mL;
ELISA: 0.2 µg/mL - 1 µg/mL;
Multiplex: 0.05 µg/mL – 0.5 µg/mL;
Immunocytochemistry: 1 µg/mL - 2 µg/mL.
Keywords
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Product Images
at Lysine 7 (K7ac). No cross reactivity with non-modified
Lysine 7 or other acetylated Lysines in histone H2A.
treated (+) or untreated (-) with sodium butyrate,
using RM222 at 0.5 µg/mL, showed a band of
histone H2A.Z acetylated at Lysine 7 in treated
HeLa.
with sodium butyrate, using anti-Acetyl-Histone
H2A.Z (Lys7) Rabbit Monoclonal Antibody, Clone
RM222 (red). Actin filaments have been labeled
with fluorescein phalloidin (green).
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