RM235 specifically reacts to Histone H2B acetylated Western Blot of acid extracts from HeLa cells Immunocytochemical staining of HeLa cells treated
Product Details
Product Name Anti-Acetyl-Histone H2B (Lys20) Rabbit Monoclonal Antibody, Clone RM235
Product Description Rabbit monoclonal to Acetyl-Histone H2B (Lys20)
Catalog No. 31-1113-00
Clone Name RM235
Product Data Sheet Anti-Acetyl-Histone H2B (Lys20) Rabbit Monoclonal Antibody Data Sheet RM235
Specificity RM235 reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys20).
Application Western Blot (WB)
ELISA
Multiplex
Immunocytochemistry (ICC)
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 µg/mL – 2 µg/mL;
ELISA: 0.2 µg/mL - 1 µg/mL;
Multiplex: 0.1 µg/mL – 0.5 µg/mL;
Immunocytochemistry: 1 µg/mL- 2 µg/mL.
Keywords
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Product Images
at Lysine 20 (K20ac). No cross reactivity with non-
modified Lysine 20 or other acetylated Lysines in
histone H2B.
treated (+) or untreated (-) with sodium butyrate,
using RM235 at 0.5 µg/mL.
with sodium butyrate, using anti-Acetyl-Histone
H2B (Lys20) Rabbit Monoclonal Antibody (clone
RM235) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).
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Anti-Acetyl-Histone H2B (Lys20) Rabbit Monoclonal Antibody, Histone H2B (Acetyl Lys20), Clone RM235