RM130 specifically reacts to Histone H3 acetylated The binding specificity of RM130 to Histone H3 Western Blot of acid extracts from HeLa cells ChIP performed on HeLa cells using H3K14ac Immunocytochemistry of HeLa cells treated with
Product Details
Product Name Anti-Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody, Clone RM130
Product Description Rabbit monoclonal to Acetylated Histone H3 Lysine 14 (K14ac), H3K14ac, Histone H3 (K14 acetylation)
Catalog No. 31-1032-00
Clone Name RM130
Product Data Sheet Anti-Acetyl-Histone H3 Lys14 Rabbit Monoclonal Antibody Data Sheet RM130
Specificity RM130 reacts to Histone H3 acetylated at Lysine 14 (K14ac), and is not affected by the modification of neighboring amino acids. No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys14)
Application Western Blot (WB)
Immunocytochemistry (ICC)
Chromatin IP (ChIP)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 µg/mL - 2 µg/mL;
ICC: 0.5 µg/mL - 2 µg/mL;
ChIP: 2 µg/mL- 10 µg/mL;
ELISA: 0.2 µg/mL - 1 µg/mL;
Multiplex: 0.1 µg/mL – 0.5 µg/mL.
Keywords
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Product Images
at Lysine 14 (K14ac). No cross reactivity with
acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine
18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac),
Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.
acetylated at Lysine 14 (K14ac) is not affected
by the modification of neighboring amino acids.
untreated (-) or treated with sodium butyrate (+)
or, using RM130 at 0.5 µg/mL.
antibody (RM130, 5 µg). Real-time PCR was
performed using primers specific to the gene
indicated.
sodium butyrate, using Acetyl-Histone H3 (Lys14)
Rabbit mAb RM130 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).
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