RM156 specifically reacts to Histone H3 acetylated Western Blot of acid extracts from HeLa cells Sandwich ELISA against acetylated histone H3 ChIP performed on HeLa cells with or without Immunocytochemistry of HeLa cells treated with
Product Details
Product Name Anti-Acetyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody, Clone RM156
Product Description Rabbit monoclonal to Acetylated Histone H3 Lysine 79 (K79ac), H3K79ac, Histone H3 (K79 acetylation)
Catalog No. 31-1052-00
Clone Name RM156
Product Data Sheet Anti-Acetyl-Histone H3 Lys79 Rabbit Monoclonal Antibody Data Sheet RM156
Specificity RM156 reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with other acetylated Lysines in Histone H3.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys79).
Application Western Blot (WB)
Chromatin IP (ChIP)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 µg/mL - 2 µg/mL;
ICC: 0.5 µg/mL - 2 µg/mL;
ChIP: 2 µg/mL- 10 µg/mL;
ELISA: 0.2 µg/mL - 1 µg/mL;
Multiplex: 0.05 µg/mL – 0.2 µg/mL.
Keywords
.
References Mondal B et al The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth. Elife. (2020), 10.7554/eLife.57519. WB; Human. Read more (PubMed: 32297854)
Product Images
at Lysine 79 (K79ac). No cross reactivity with acetyl-
ated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14
(K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac),
Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56
(K56ac), or Lysine 122 (K122) in Histone H3.
untreated (-) or treated with sodium butyrate (+),
using RM156 at 1.0 µg/mL, showed a band of
histone H3 acetylated at Lysine 79 in treated
HeLa.
at Lys 79 using HeLa whole cell lysate, treated or
untreated with Sodium Butyrate. Using anti-H3K79ac
(RM156, 5 µg/mL) as the capture antibody and
biotinylated anti-H3CT (RM188, 1 µg/mL) as the
detection antibody.
Sodium Butyrate treatment, using H3K79Ac
antibody (RM156, 5 µg). Real-time PCR was
performed using primers specific to the gene
indicated.
sodium butyrate, using Acetyl-Histone H3 (Lys79)
Rabbit mAb RM156 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).
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