Immunocytochemical staining of HeLa cells using Western blot of HeLa cells nontreated or treated Immunoprecipitation of A431 cell lysates, using RevMAb rabbit monoclonal antibody Clone
Product Details
Product Name Anti-Acetylated-Lysine Rabbit Monoclonal Antibody, Clone RM101
Description Rabbit monoclonal to Acetylated-Lysine
Catalog No. 32-1012-00
Clone Name RM101
Product Data Sheet Anti-Acetylated Lysine Rabbit Monoclonal Antibody Data Sheet RM101
Specificity RM101 reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, and lysines with other modifications.
Immunogen Acetyllysine-BSA
Application Western Blot (WB)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species Reactivity All Species
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot (WB): 1:500 -1:2000 dilution;
Immunoprecipitation (IP): 1:100-1:500 dilution;
Chromatin IP (ChIP): 1:100-1:500 dilution;
Immunocytochemistry (ICC): 1:100-1:500 dilution;
Immunohistochemistry (IHC): 1:100-1:500 dilution.
Keywords
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Product Images
Anti-Acetylated Lysine antibody (Clone RM101) (red).
Actin filaments was labeled with fluorescein phalloidin
(green), and nucleus stained with DAPI (blue).
with Trichostatin A (TSA), using RevMAb Clone
RM101 at 1/2000 dilution.
RevMAb Clone RM101 at 1/500 dilution, was
blotted with anti-PTEN mouse monoclonal antibody.
RM101 recognizes acetylated lysine in peptides
with different sequences. It does not react with
non-acetylated lysine.
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