Competitive ELISA of cGMP using anti-cGMP (RM467). The 96-well plate was coated Competitive ELISA showing the specificity of anti-cGMP (RM467). The 96-well Competitive ELISA detecting cGMP in HeLa cells. The 96-well plate was coated with
Product Details
Product Name Anti-cGMP Rabbit Monoclonal Antibody, Clone RM467
Product Description Rabbit monoclonal to cGMP (Cyclic Guanosine Monophosphate)
Catalog No. 31-1359-00
Clone Name RM467
Product Data Sheet Anti-cGMP Rabbit Monoclonal Antibody Data Sheet RM467
Specificity RM467 reacts to cGMP (Cyclic Guanosine Monophosphate). No cross reactivity with other cyclic nucleotide or nucleoside phosphate.
Immunogen KLH-conjugated cGMP
Application ELISA
Conjugate None
Intended Use Research Use Only
Related Product Antigen for Competitive Assay RM-PA467 (71-1359-00) - cGMP-HRP
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50 µg
Concentration 1 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.05 µg/mL – 0.5 µg/mL
Keywords
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Product Images
with 1 µg/mL of Goat anti-rabbit IgG (50 µL/well). 0.05 µg/ml of anti-cGMP (RM467)
(50 µL/well) was added and incubated. After wash and block, different concentrations of
cGMP (25 µL/well) were added along with 25 µL/well of 1/5,000 diluted HRP conjugated cGMP
(RM-PA467 (71-1359-00)). TMB was used to develop the color after incubation and wash.
plate was coated with 1 µg/mL of Goat anti-rabbit IgG (50 µL/well). 0.05 µg/ml
of anti-cGMP (RM467) (50 µL/well) was added and incubated. After wash
and block, cGMP and other cyclic nucleotides or nucleoside phosphates
(25 µL/well) were added along with 25 µL/well of 1/5,000 diluted HRP
conjugated cGMP (RM-PA467 (71-1359-00)). TMB was used to develop
the color after incubation and wash.
1 µg/mL of Goat anti-rabbit IgG (50 µL/well). 0.05 µg/mL of anti-cGMP (RM467)
(50 µL/well) was added and incubated. After wash and block, HeLa cell lysate
(~105 cell/mL) or control (lysate buffer only) (25 µL/well) were added along with
25 µL/well of 1/5,000 diluted HRP conjugated cGMP (RM-PA467 (71-1359-00)).
TMB was used to develop the color after incubation and wash.
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