Western Blot of acid extracts of HeLa cells with or without sodium crotonate treatment using Anti-Histone H3K27cr Rabbit Monoclonal Antibody (Clone RM401) at 0.5 µg/mL. A Peptide dotblot showing Anti-Histone H3K27cr Rabbit Monoclonal Antibody (Clone RM401) Image courtesy of Jibo Zhang from Strahl lab,
Product Details
Product Name Anti-Crotonyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM401
Product Description Rabbit monoclonal to Crotonyl-Histone H3 (Lys27).
Catalog No. 31-1287-00
Clone Name RM401
Product Data Sheet Anti-Crotonyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody Data Sheet RM401
Specificity RM401 reacts to Histone H3 crotonylated at Lysine 27 (K27cr). No cross reactivity with other crotonylated or acetylated Lysines in histone H3.
Immunogen A peptide corresponding to Crotonyl-Histone H3 (Lys27).
Application Western Blot (WB)
Dot Blot (DB)
Species Reactivity Human
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage WB: 0.5 µg/mL - 5 µg/mL;
DB: 0.5 µg/mL - 2 µg/mL;
Keywords
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Product Images
reacts specifically to Histone H3 crotonylated at Lysine27 (H3K27-Crotonyl).
UNC School of Medicine
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