A Peptide dotblot shows RM168 only reacts to Histone. RM168 specifically reacts to Histone H3 dimethylated Western Blot of recombinant histone H3.3 (1)
Product Details
Product Name Anti-Dimethyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody, Clone RM168
Product Description Rabbit monoclonal to Dimethylated Histone H3 Lysine 18, H3K18me2, Histone H3 (dimethyl K18)
Catalog No. 31-1075-00
Clone Name RM168
Product Data Sheet Anti-Dimethyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody Data Sheet RM168
Specificity RM168 reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with nonmodified Lysine 18, monomethylated Lysine 18 (K18me1), or other methylations in histone H3.
Immunogen A dimethyl-peptide corresponding to dimethyl-Histone H3 (Lys18).
Application Western Blot (WB)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.2 µg/mL – 1 µg/mL;
ELISA: 0.1 µg/mL - 0.5 µg/mL;
Multiplex: 0.1 µg/mL – 0.5 µg/mL.
Keywords
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Product Images
H3 dimethyl-Lysine 18 (K18me2). No cross reactivity.
with nonmodified Lysine 18 (K18Ctrl), monomethylated.
Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2),.
or dimethylated Lysine 36 (K36me2).
at Lysine 18 (K18me2). No cross reactivity with other
methylated lysines in Histone H3.
and acid extracts of HeLa cells (2), using RM168
at 0.5 µg/mL, showed a band of histone H3
dimethylated at Lysine 18 (K18me2) in HeLa cells.
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