A Peptide dotblot shows RM171 only reacts to Histone. RM171 specifically reacts to Histone H3 dimethylated Western Blot of recombinant histone H3.3 (1)
Product Details
Product Name Anti-Dimethyl-Histone H3 (Lys23) Rabbit Monoclonal Antibody, Clone RM171
Product Description Rabbit monoclonal to Dimethylated Histone H3 Lysine 23, H3K23me2, Histone H3 (dimethyl K23)
Catalog No. 31-1077-00
Clone Name RM171
Product Data Sheet Anti-Dimethyl-Histone H3 (Lys23) Rabbit Monoclonal Antibody Data Sheet RM171
Specificity RM171 reacts to Histone H3 dimethylated at Lysine 23 (K23me2). No cross reactivity with nonmodified Lysine 23, monomethylated Lysine 23 (K23me1), or other methylations in histone H3.
Immunogen A dimethyl-peptide corresponding to dimethyl-Histone H3 (Lys23).
Application Western Blot (WB)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 µg/mL – 2 µg/mL;
ELISA: 0.1 µg/mL - 0.5 µg/mL;
Multiplex: 0.1 µg/mL – 0.5 µg/mL.
Keywords
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Product Images
H3 dimethyl-Lysine 23 (K23me2). No cross reactivity.
with nonmodified Lysine 23 (K23Ctrl), monomethylated.
Lysine 23 (K23me1), or dimethylated Lysine 4 (K4me2).
at Lysine 23 (K23me2). No cross reactivity with other
methylated lysines in Histone H3.
and acid extracts of HeLa cells (2), using RM171
at 0.5 µg/mL, showed a band of histone H3
dimethylated at Lysine 23 (K23me2) in HeLa cells.
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