RM135 specifically reacts to Histone H3 dimethylated Western Blot of recombinant histone H3.3 (1) and Immunocytochemistry of HeLa cells treated with
Product Details
Product Name Anti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM135
Product Description Rabbit monoclonal to Dimethylated Histone H3 Lysine 4, H3K4me2, Histone H3 (dimethyl K4)
Catalog No. 31-1037-00
Clone Name RM135
Product Data Sheet Anti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM135
Specificity RM135 reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with monomethylated Lysine 4 (K4me1), trimethylated Lysine 4 (K4me3), or other methylations in histone H3.
Immunogen A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys4).
Application Chromatin IP (ChIP)
Western Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ChIP: 1 µg - 5 µg;
Western Blot: 0.02 µg/mL – 0.2 µg/mL;
ICC: 1 µg/mL - 2 µg/mL;
ELISA: 0.2 µg/mL - 1 µg/mL;
Multiplex: 0.1 µg/mL – 0.5 µg/mL.
Keywords
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Product Images
at Lysine 4 (K4me2). No cross reactivity with mono-
methylated Lysine 4 (K4me1), trimethylated Lysine 4
(K4me3), or other methylations in histone H3.
acid extracts of HeLa cells (2), using RM135 at 0.025
µg/mL, showed a band of histone H3 dimethylated
at Lysine 4 (K4me2) in HeLa cells.
sodium butyrate, using Dimethyl-Histone H3 (Lys4)
Rabbit mAb RM135 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).
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