RM151 specifically reacts to Histone H3 dimethylated Western Blot of recombinant histone H3.3 (1) ChIP performed on HeLa cells using H3K9me2 Immunocytochemistry of HeLa cells treated with
Product Name Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [RM151]
Product Description Rabbit monoclonal to Dimethylated Histone H3 Lysine 9, H3K9me2, Histone H3 (dimethyl K9)
Catalog No. 31-1059-00
Clone Name RM151
Product Data Sheet Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody Data Sheet RM151
Specificity RM151 reacts to Histone H3 dimethylated at Lysine 9 (K9me2). No cross reactivity with monomethylated Lysine 9 (K9me1), trimethylated Lysine 9 (K9me3), or other methylations in histone H3.
Immunogen A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys9).
Application Western Blot (WB)
Chromatin IP (ChIP)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt.
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.25 ug/mL – 1 ug/mL;
ChIP: 2 ug/mL- 10 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
References for Dimethyl-Histone H3 (Lys9) antibody [RM151] Xiong H et al. Inhibition of Histone Methyltransferase G9a Attenuates Noise-Induced Cochlear Synaptopathy and Hearing Loss. J Assoc Res Otolaryngol. (2019), 10.1007/s10162-019-00714-6. ICC, IHC; Human. Read more (PubMed: 30710318)
. Product Images
at Lysine 9 (K9me2). No cross reactivity with non
-modified Lysine 9 (H3 1-19), monomethylated
Lysine 9 (K9me1), trimethylated Lysine 9
(K9me3), or other methylations in Histone H3.
and acid extracts of HeLa cells (2), using RM151
at 0.5 ug/mL, showed a band of histone H3
dimethylated at Lysine 9 (K9me2) in HeLa cells.
antibody (RM151, 5ug). Real-time PCR was
performed using primers specific to the gene
sodium butyrate, using Dimethyl-Histone H3
(Lys9) Rabbit mAb RM151 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).
RM151 specifically reacts to Histone H3 dimethylated
Western Blot of recombinant histone H3.3 (1)
ChIP performed on HeLa cells using H3K9me2
Immunocytochemistry of HeLa cells treated with