Western Blot of acid extracts of HeLa cells treated Immunocytochemistry of HeLa cells using gamma
Product Details
Product Name Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody, Clone RM224
Product Description Rabbit monoclonal to Gamma H2AX (phospho-Ser139)
Catalog No. 31-1104-00
Clone Name RM224
Product Data Sheet Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody Data Sheet RM224
Specificity RM224 reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.
Immunogen A phospho-peptide corresponding to Phospho-Histone H2AX (Ser139).
Application Western Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt.
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 μg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 μg/mL – 2 μg/mL;
ELISA: 0.2 μg/mL - 1 μg/mL;
Multiplex: 0.1 μg/mL – 1 μg/mL;
ICC: 0.5 μg/mL - 2 μg/mL.
Keywords
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Product Images
or non-treated with UV. Using RM224 at 0.5 ug/mL,
showed a band of Histone H2A.X phosphorylated
at serine 139 in HeLa cells.
H2AX Rabbit mAb RM224 (red). Actin filaments
have been labeled with fluorescein phalloidin (green).
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