Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146X

Name: Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146X
SKU: 38-1048-00
Price: 386.00 USD
Availability: InStock

$386.00

Rabbit monoclonal to His-Tag at either the N-Terminus (amino) or C-Terminus (carboxy)

For N-terminal His-tagged protein detection, RM146X is recommended.

100 µg

SKU: 38-1048-00 Categories: Epitope Tags, Primary Antibodies, Rabbit Monoclonal Tags: 38-1048-00, 38104800

Description
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Product Details
Product Name	Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146X
Product Description	Rabbit monoclonal to His-Tag at either the N-Terminus (amino) or C-Terminus (carboxy)
Catalog No.	38-1048-00
Clone Name	RM146X
Product Data Sheet	Anti-His Tag Rabbit Monoclonal Antibody Data Sheet RM146X
Specificity	RM146X reacts to recombinant proteins containing the His-Tag fused to either the amino or carboxy terminus. Very low non-specific reactivity with endogenous proteins in mammalian or bacteria cells, compared with other anti-HisTag antibodies.
Immunogen	6xHis-Tag coupled to KLH
Application	Western Blot ELISA Immunoprecipitation Immunocytochemistry
Species Reactivity	All
Conjugate	None
Intended Use	Research Use Only
Related Products	Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 µg
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	WB: 0.01 µg/mL – 0.1 µg/mL; ELISA: 0.01 µg/mL – 0.2 µg/mL; IP: 0.5 µg/mL – 2 µg/mL; FFPE ICC: 0.5 µg/mL – 2 µg/mL. IHC: 0.004 µg/mL – 0.1 µg/mL
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Product Images
	Western blot of 293 cell or E. coli whole cell lysate with or without N-terminal His tagged fusion protein X or C-terminal His tagged fusion protein Y, using anti-HisTag rabbit monoclonal antibody, Clone RM146X (0.02 µg/mL).
	ELISA comparison of a N-terminal His tagged fusion protein (50 µg/well) and a C-terminal His tagged fusion protein (50 µg/well), using our anti-His Tag rabbit monoclonal antibody, Clone RM146X, versus a leading anti-HisTag mouse monoclonal antibody.
	Western blots showing non-specific reactivities of anti-HisTag rabbit monoclonal antibody, Clone RM146X with endogenous proteins in mammalian 293 cells bacteria cells, compared with a leading anti-HisTag mouse monoclonal antibody.
	Western blots showing non-specific reactivities of anti-HisTag rabbit monoclonal antibody, Clone RM146X with endogenous proteins in mammalian E. coli bacteria cells, compared with a leading anti-HisTag mouse monoclonal antibody.
	Immunostaining of FFPE 293 cells overexpressing a cytoplasmic or nuclear protein, using anti-His-tag rabbit monoclonal antibody, Clone RM146X, at 4 ng/mL