Western blot of 293 cell or E. coli whole cell lysate with or without N-terminal ELISA comparison of a N-terminal His tagged fusion protein (50 µg/well) Western blots showing non-specific reactivities of anti-HisTag rabbit monoclonal Western blots showing non-specific reactivities of anti-HisTag rabbit monoclonal Immunostaining of FFPE 293 cells overexpressing a
Product Details
Product Name Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146X
Product Description Rabbit monoclonal to His-Tag at either the N-Terminus (amino) or C-Terminus (carboxy)
Catalog No. 38-1048-00
Clone Name RM146X
Product Data Sheet Anti-His Tag Rabbit Monoclonal Antibody Data Sheet RM146X
Specificity RM146X reacts to recombinant proteins containing the His-Tag fused to either the amino or carboxy terminus. Very low non-specific reactivity with endogenous proteins in mammalian or bacteria cells, compared with other anti-HisTag antibodies.
Immunogen 6xHis-Tag coupled to KLH
Application Western Blot
ELISA
Immunoprecipitation
Immunocytochemistry
Species Reactivity All
Conjugate None
Intended Use Research Use Only
Related Products Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage WB: 0.01 µg/mL – 0.1 µg/mL;
ELISA: 0.01 µg/mL – 0.2 µg/mL;
IP: 0.5 µg/mL – 2 µg/mL;
FFPE ICC: 0.5 µg/mL – 2 µg/mL.
IHC: 0.004 µg/mL – 0.1 µg/mL
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Product Images
His tagged fusion protein X or C-terminal His tagged fusion protein Y,
using anti-HisTag rabbit monoclonal antibody, Clone RM146X (0.02 µg/mL).
and a C-terminal His tagged fusion protein (50 µg/well), using our anti-His
Tag rabbit monoclonal antibody, Clone RM146X, versus a leading anti-HisTag
mouse monoclonal antibody.
antibody, Clone RM146X with endogenous proteins in mammalian 293 cells bacteria cells, compared with a leading anti-HisTag mouse monoclonal antibody.
antibody, Clone RM146X with endogenous proteins in mammalian E. coli bacteria cells,
compared with a leading anti-HisTag mouse monoclonal antibody.
cytoplasmic or nuclear protein, using anti-His-tag rabbit
monoclonal antibody, Clone RM146X, at 4 ng/mL
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Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146X
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Rabbit monoclonal to His-Tag at either the N-Terminus (amino) or C-Terminus (carboxy)
For N-terminal His-tagged protein detection, RM146X is recommended.
100 µg
SKU: 38-1048-00
Categories: Epitope Tags, Primary Antibodies, Rabbit Monoclonal
Tags: 38-1048-00, 38104800
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