Anti-Histone H3.3 G34R Rabbit Monoclonal Antibody, Clone RM240; Histone H3.3 G34R Mutant (50ug)

$375.00

Rabbit monoclonal to Histone H3.3 G34R; Histone H3.3 G34R Mutant

Product Details
Product NameAnti-Histone H3.3 G34R Rabbit Monoclonal Antibody [RM240]
Product DescriptionRabbit monoclonal to Histone H3.3 G34R; Histone H3.3 G34R Mutant
Catalog No.31-1120-00
Clone NameRM240
Product Data SheetAnti-Histone H3.3 G34R Mutant Rabbit Monoclonal Antibody Data Sheet RM240
SpecificityRM240 reacts to the Histone H3.3 G34R mutant. No cross reactivity with wild type Histone H3.3 .
ImmunogenA peptide corresponding to Histone H3.3 G34R mutant
ApplicationWestern Blot
ELISA
Immunohistochemistry
Immunocytochemistry
Chromatin IP
Species ReactivityAll
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size50 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.5 ug/mL - 2 ug/mL;
Immunohistochemistry (IHC): 0.5 – 5 ug/mL;
Immunocytochemistry (ICC): 0.5 – 5 ug/mL.
References for Histone H3.3 G34R Mutant antibody [RM240]Pathania M et al. H3.3K27M Cooperates with Trp53 Loss and PDGFRA Gain in Mouse Embryonic Neural Progenitor Cells to Induce Invasive High-Grade Gliomas Cancer Cell (2017) 10.1016/j.ccell.2017.09.014. IHC-P; Human. Read more (PubMed: 29107533)

Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742)
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Product Images
Western

Western Blot analysis of cell lysates prepared from
293T, transfected with a DNA construct encoding
G34R mutant or wild type proteins of Histone H3.3,
using anti-Histone H3.3 G34R clone RM240.

Immunohistochemical

Immunohistochemical staining of formalin fixed and
paraffin embedded 293T cells transfected with a DNA
construct encoding Histone H3.3 G34R mutant or wild
type, stained with anti-Histone H3.3 G34R clone RM240.

IHC

IHC of clinically validated FFPE Glioblastoma tumor
tissues with H3.3 G34R expression, using anti-histone
H3.3 G34R rabbit monoclonal antibody, Clone RM240.
Clinically validated to be Positive for H3.3 G34R Mutation.



Image courtesy of Dr. Sebastian Brandner, UCL
Institute of Neurology, London, United Kingdom

IHC

IHC of clinically validated FFPE Glioblastoma tumor
tissues, using anti-histone H3.3 G34R rabbit
monoclonal antibody, Clone RM240. Clinically validated
to be Negative for H3.3 G34R Mutation.



Image courtesy of Dr. Sebastian Brandner, UCL
Institute of Neurology, London, United Kingdom

ICC staining of primary human cell lines expressing
WT and H3.3 G34R Mutation.


Image courtesy of Dr. Nada Jabado, McGill University,
Montreal, Quebec, Canada.



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References

Pathania M et al. H3.3K27M Cooperates with Trp53 Loss and PDGFRA Gain in Mouse Embryonic Neural Progenitor Cells to Induce Invasive High-Grade Gliomas Cancer Cell (2017) 10.1016/j.ccell.2017.09.014. IHC-P; Human. Read more (PubMed: 29107533)

Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742)