Western Blot analysis of cell lysates prepared from IHC of clinically validated FFPE Glioblastoma tumor Image courtesy of Dr. Sebastian Brandner, UCL IHC of clinically validated FFPE Glioblastoma tumor Image courtesy of Dr. Sebastian Brandner, UCL ICC staining of primary human cell lines expressing Image courtesy of Dr. Nada Jabado, McGill University, Montreal, Quebec, Canada.
Product Details
Product Name Anti-Histone H3.3 G34R Rabbit Monoclonal Antibody, Clone RM240
Product Description Rabbit monoclonal to Histone H3.3 G34R; Histone H3.3 G34R Mutant
Catalog No. 31-1120-00-S;
31-1120-00-L;
31-1120-02
Clone Name RM240
Product Data Sheet Anti-Histone H3.3 G34R Mutant Rabbit Monoclonal Antibody Data Sheet RM240
Biotinylated Anti-Histone H3.3 G34R Mutant Rabbit Monoclonal Antibody Data Sheet RM240
Specificity RM240 reacts to the Histone H3.3 G34R mutant. No cross reactivity with wild type Histone H3.3 .
Immunogen A peptide corresponding to Histone H3.3 G34R mutant
Application Western Blot
ELISA
Immunohistochemistry
Chromatin IP
Species Reactivity All
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µL;
400 µL;
Biotinylated 50 µL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 1:200 – 1:1000;
ELISA: 1:250 – 1:2500;
IHC: 1:100 – 1:500.
References for Histone H3.3 G34R Mutant antibody [RM240] Nambiraja A et al C19MC amplification and expression of Lin28A and Olig2 in the classification of embryonal tumors of the central nervous system: A 14-year retrospective study from a tertiary care center. Childs Nerv Syst. (2021), 10.1007/s00381-020-04973-0. IHC; Human. Read more (PubMed: 33236184)
Esteve-Codina A et al RNA sequencing and Immunohistochemistry Reveal ZFN7 as a Stronger Marker of Survival than Molecular Subtypes in G-CIMP-negative Glioblastoma. Clin Cancer Res. (2021), 10.1158/1078-0432.CCR-20-2141. IHC; Human. Read more (PubMed: 33106291)
Bressa RB et al Regional identity of human neural stem cells determines oncogenic responses to histone H3.3 mutants. Cell Stem Cell. (2021), 10.1016/j.stem.2021.01.016. IHC; Human. Read more (PubMed: 33631116)
Minasi S et al Alternative lengthening of telomeres in molecular subgroups of paediatric high-grade glioma. Childs Nerc Syst. (2020), 10.1007/s00381-020-04933-8. IHC; Human. Read more (PubMed: 33128602)
Pathania M et al. H3.3K27M Cooperates with Trp53 Loss and PDGFRA Gain in Mouse Embryonic Neural Progenitor Cells to Induce Invasive High-Grade Gliomas Cancer Cell (2017) 10.1016/j.ccell.2017.09.014. IHC-P; Human. Read more (PubMed: 29107533)
Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742)
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Product Images
293T, transfected with a DNA construct encoding
G34R mutant or wild type proteins of Histone H3.3,
using anti-Histone H3.3 G34R, Clone RM240.
tissues with H3.3 G34R expression, using anti-histone
H3.3 G34R rabbit monoclonal antibody, Clone RM240.
Clinically validated to be Positive for H3.3 G34R Mutation.
Institute of Neurology, London, United Kingdom
tissues, using anti-histone H3.3 G34R rabbit
monoclonal antibody, Clone RM240. Clinically validated
to be Negative for H3.3 G34R Mutation.
Institute of Neurology, London, United Kingdom
WT and H3.3 G34R Mutation.
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