RM190 reacts specifically to Histone H3.3. Western Blot of HeLa whole cell lysate,
Product Details
Product Name Anti-Histone H3.3 Rabbit Monoclonal Antibody, Clone RM190
Product Description Rabbit monoclonal to Histone H3.3
Catalog No. 31-1058-00
Clone Name RM190
Product Data Sheet Anti-Histone H3.3 Rabbit Monoclonal Antibody Data Sheet RM190
Specificity RM190 reacts to Histone H3.3, independent of post-translational modifications. No cross reactivity with Histone H3.1 or other histone proteins.
Immunogen A peptide corresponding to human Histone H3.3.
Application Western Blot (WB)
ELISA
Multiplex
Immunofluorescence (IF)
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 µg/mL – 2 µg/mL;
ELISA: 0.2 µg/mL - 1 µg/mL;
Multiplex: 0.05 µg/mL – 0.5 µg/mL.
Keywords
References for Histone H3.3 Rabbit Monoclonal Antibody [RM190] Hinchcliffe EH et al. Chromosome missegregation during anaphase triggers p53 cell cycle arrest through histone H3.3 Ser31 phosphorylation Nat Cell Biol. (2016) 10.1038/ncb3348. IF; Human, Monkey. Read more (PubMed: 27136267)
Product Images
No cross reactivity with Histone H3.1.
recombinant Histone H3.3 and Histone H3.1
proteins, using RM190 at 1 µg/mL, showed
a band of histone H3.3 in HeLa cells and
recombinant H3.3 protein, and no cross
activity with Histone H3.1.
Related Products
Anti-Histone H3.3 Rabbit Monoclonal Antibody, Clone RM190
$198.00 – $441.00
Rabbit monoclonal Histone H3.3
25 µg Trial Size, 100 µg Full Size
SKU: 31-1058-00
Categories: Histone Family Proteins and Variants, Histone Modification, Primary Antibodies, Rabbit Monoclonal
Tags: 31-1058-00, 31-1058-00-S, 31105800, 31105800S
Size Format | 25 µg, 100 µg |
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References
Hinchcliffe EH et al. Chromosome missegregation during anaphase triggers p53 cell cycle arrest through histone H3.3 Ser31 phosphorylation Nat Cell Biol. (2016) 10.1038/ncb3348. IF; Human, Monkey. Read more (PubMed: 27136267)