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Anti-Histone H3 K27M Rabbit Monoclonal Antibody, Clone RM192; Histone H3, K27M Mutant (50ug)

$375.00

Rabbit monoclonal to Histone H3 K27M; Histone H3, K27M Mutant

SKU: 31-1175-00. Categories: , , , , . Tags: , .

Product Details
Product NameAnti-Histone H3 K27M Rabbit Monoclonal Antibody [RM192]
Product DescriptionRabbit monoclonal to Histone H3 K27M; Histone H3, K27M Mutant
Catalog No.31-1175-00
Clone NameRM192
Product Data SheetAnti-Histone H3 K27M Rabbit Monoclonal Antibody Data Sheet RM192
SpecificityRM192 reacts to the Histone H3 K27M mutant. No cross reactivity with wild type Histone H3.
ImmunogenA peptide corresponding to Histone H3 K27M mutant
ApplicationWestern Blot
ELISA
Immunohistochemistry
Immunocytochemistry
Species ReactivityAll
ConjugateNone
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size50 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.01 ug/mL – 1 ug/mL;
ELISA: 0.5 ug/mL - 2 ug/mL;
Immunohistochemistry (IHC): 0.005 – 0.1 ug/mL;
Immunocytochemistry (ICC/IF): 0.005 – 0.1 ug/mL.
References for Histone H3 K27M Mutant antibody [RM192]Pathania M et al. H3.3K27M Cooperates with Trp53 Loss and PDGFRA Gain in Mouse Embryonic Neural Progenitor Cells to Induce Invasive High-Grade Gliomas Cancer Cell (2017) 10.1016/j.ccell.2017.09.014. IHC-P; Human. Read more (PubMed: 29107533)
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Product Images

Immunostaining of brain tumor tissue sections
 using both EDTA and Citrate pretreatment methods.
Clinically validated to be Positive for the H3.3 K27M
Mutation by Sanger sequencing.


Image courtesy of Sebastian Brandner MD, Division
 of Neuropathology and Dept. of Neurodegenerative Disease,
 UCL Institute of Neurology, London, UK

Immunostaining of brain tumor tissue sections
using both EDTA and Citrate pretreatment methods.
Clinically validated to be Negative for the H3.3 K27M
Mutation by Sanger sequencing.


Image courtesy of Sebastian Brandner MD, Division
 of Neuropathology and Dept. of Neurodegenerative Disease,
 UCL Institute of Neurology, London, UK

Western

Western Blot analysis of cell lysates prepared from
 293T transfected with a DNA construct encoding
 wild type (WT) or K27M mutant proteins of Histone
H3.3, using 0.01 ug/mL of anti-Histone H3 K27M
rabbit monoclonal antibody clone RM192.

Immunohistochemical

Immunohistochemical staining of formalin fixed
and paraffin embedded 293T cells transfected
with a DNA construct encoding Histone H3 K27M
mutant, stained with 0.01 ug/mL of anti-Histone H3
 K27M rabbit monoclonal antibody clone RM192.


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References

Pathania M et al. H3.3K27M Cooperates with Trp53 Loss and PDGFRA Gain in Mouse Embryonic Neural Progenitor Cells to Induce Invasive High-Grade Gliomas Cancer Cell (2017) 10.1016/j.ccell.2017.09.014. IHC-P; Human. Read more (PubMed: 29107533)