Immunostaining of brain tumor tissue sections Image courtesy of Sebastian Brandner MD, Division Immunostaining of brain tumor tissue sections Image courtesy of Sebastian Brandner MD, Division Western Blot analysis of cell lysates prepared from Immunohistochemical staining of formalin fixed
Product Details
Product Name Anti-Histone H3 K27M Rabbit Monoclonal Antibody [RM192]
Product Description Rabbit monoclonal to Histone H3 K27M; Histone H3, K27M Mutant
Catalog No. 31-1175-00
Clone Name RM192
Product Data Sheet Anti-Histone H3 K27M Rabbit Monoclonal Antibody Data Sheet RM192
Specificity RM192 reacts to the Histone H3 K27M mutant. No cross reactivity with wild type Histone H3.
Immunogen A peptide corresponding to Histone H3 K27M mutant
Application Western Blot
ELISA
Immunohistochemistry
Immunocytochemistry
Species Reactivity All
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 50 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.01 ug/mL – 1 ug/mL;
ELISA: 0.5 ug/mL - 2 ug/mL;
Immunohistochemistry (IHC): 0.005 – 0.1 ug/mL;
Immunocytochemistry (ICC/IF): 0.005 – 0.1 ug/mL.
References for Histone H3 K27M Mutant antibody [RM192] Pathania M et al. H3.3K27M Cooperates with Trp53 Loss and PDGFRA Gain in Mouse Embryonic Neural Progenitor Cells to Induce Invasive High-Grade Gliomas Cancer Cell (2017) 10.1016/j.ccell.2017.09.014. IHC-P; Human. Read more (PubMed: 29107533)
.
Product Images
using both EDTA and Citrate pretreatment methods.
Clinically validated to be Positive for the H3.3 K27M
Mutation by Sanger sequencing.
of Neuropathology and Dept. of Neurodegenerative Disease,
UCL Institute of Neurology, London, UK
using both EDTA and Citrate pretreatment methods.
Clinically validated to be Negative for the H3.3 K27M
Mutation by Sanger sequencing.
of Neuropathology and Dept. of Neurodegenerative Disease,
UCL Institute of Neurology, London, UK
293T transfected with a DNA construct encoding
wild type (WT) or K27M mutant proteins of Histone
H3.3, using 0.01 ug/mL of anti-Histone H3 K27M
rabbit monoclonal antibody clone RM192.
and paraffin embedded 293T cells transfected
with a DNA construct encoding Histone H3 K27M
mutant, stained with 0.01 ug/mL of anti-Histone H3
K27M rabbit monoclonal antibody clone RM192.
Related Products
Home/Primary Antibodies/Rabbit Monoclonal/Epigenetics/Histone Mutations
Anti-Histone H3 K27M Rabbit Monoclonal Antibody, Clone RM192; Histone H3, K27M Mutant (50ug)
$399.00
Rabbit monoclonal to Histone H3 K27M; Histone H3, K27M Mutant
References
Pathania M et al. H3.3K27M Cooperates with Trp53 Loss and PDGFRA Gain in Mouse Embryonic Neural Progenitor Cells to Induce Invasive High-Grade Gliomas Cancer Cell (2017) 10.1016/j.ccell.2017.09.014. IHC-P; Human. Read more (PubMed: 29107533)