Immunohistochemistry of Human Tonsil using ELISA of human immunoglobulins shows RM116 reacted A titer ELISA using RM116. The plate was coated Sandwich ELISA using RM116 as the capture antibody Sandwich ELISA using RM116 as the capture antibody Cross-activity data of RevMAb anti-human IgG antibodies against
Product Details
Product Name Anti-Human Gamma Heavy Chain Rabbit Monoclonal Antibody, Clone RM116
Product Description Rabbit Monoclonal to Human Gamma Heavy Chain
Catalog No. 31-1018-00
Clone Name RM116
Product Data Sheet Anti-Human Gamma Heavy Chain Rabbit Monoclonal Antibody Data Sheet RM116
Specificity RM116 reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including γ1, γ2, γ3, and γ4. No cross reactivity with human IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Immunogen Human IgG
Application ELISA
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
Species Reactivity Human
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL;
Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL.
Keywords
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Product Images
Anti-Gamma Heavy Chain antibody, Clone RM116.
to the γ1, γ2, γ3, γ4 heavy chain of human IgGs, and the
Fc of human IgG. No cross reactivity with other human
heavy chains, mouse IgG, rat IgG, or goat IgG. The plate
was coated with 50 ng/well of different immunoglobulins.
200 ng/mL or 50 ng/mL of RM116 was used as the primary
antibody. An alkaline phosphatase conjugated anti-rabbit IgG
as the secondary antibody.
with different amounts of human IgG1. A serial dilution
of RM116 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.
(100 ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody (1 µg/mL),
followed by an alkaline phosphatase conjugated streptavidin.
(100 ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody (1 µg/mL),
followed by an alkaline phosphatase conjugated streptavidin.
monkey IgG. ELISA plate was coated with 0.1 µg/well (2 µg/mL,
50 µL/well) of Rhesus monkey IgG (protein A purified from serum).
A serial dilution of anti-human IgG (RM116), anti-human IgG1 (RM117),
anti-human IgG2 (RM118), anti-human IgG3 RM119), anti-human
IgG4 (RM120), anti-human kappa (RM126), and anti-human lambda
(RM127) was used as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.
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