ELISA shows that clone RM129 does not react to ELISA shows that clone RM129 reacts only to A titer ELISA using RM129. The plate was coated with A titer ELISA using RM129. The plate was coated with
Product Details
Product Name Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129
Product Description Rabbit monoclonal to Human Immunoglobulin Light Chains
Catalog No. 32-1031-00
Clone Name RM129
Product Data Sheet Anti-Human Ig Light Chain Rabbit Monoclonal Antibody Data Sheet RM129
Specificity RM129 reacts to both kappa and lambda light chain of human immunoglobulins. It does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Immunogen Human IgG
Application ELISA
Immunohistochemistry (IHC)
Immunocytochemistry (ICC)
Flow Cytometry (FC)
Species Reactivity Human
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Keywords
Usage ELISA: 0.1 µg/mL - 0.5 µg/mL;
Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL;
Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL.;
Flow Cytometry (FC): 0.5 µg/mL - 2 µg/mL.
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Product Images
monkey IgG. The plate was coated with Rhesus
monkey IgG. A serial dilution of RM129 and a
monkey IgG reacting antibody (positive control)
was used as the detection antibodies.
the kappa and lambda light chains in all human
immunoglobulins, and does not react to mouse
IgG, rat IgG, or goat IgG.
different amounts of human IgG4k. A serial dilution of
RM129 was used as the primary antibody, followed by
an alkaline phosphatase conjugated anti-rabbit IgG
as the secondary antibody.
different amounts of human IgG3λ. A serial dilution of
RM129 was used as the primary antibody, followed by
an alkaline phosphatase conjugated anti-rabbit IgG
as the secondary antibody.
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