Immunohistochemistry of Human Tonsil Tissue using Sandwich ELISA using RM124 as the capture antibody ELISA of human immunoglobulins shows RM124 reacts A titer ELISA using RM124. The plate was coated with
Product Details
Product Name Anti-Human IgA1 Rabbit Monoclonal Antibody, Clone RM124
Product Description Rabbit monoclonal to Human IgA1
Catalog No. 31-1026-00
Clone Name RM124
Product Data Sheet Anti-Human IgA1 Rabbit Monoclonal Antibody Data Sheet RM124
Specificity RM124 reacts to human IgA1, and very slightly cross reacts with IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.
Immunogen Human IgA
Application ELISA
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
Species Reactivity Human
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL;
Immunohistochemistry (IHC): 0.1 µg/mL - 1 µg/mL.
Keywords
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Product Images
Anti-Human IgA1 antibody, Clone RM124.
(100 ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.
only to Human IgA. Very slightly cross reacts with IgA2.
No cross reactivity with Human IgG, IgM, IgD, or IgE.
The plate was coated with 50 ng/well of different
immunoglobulins. 200 ng/mL, 50 ng/mL,
or 10 ng/mL of RM124 was used as the primary antibody.
An alkaline phosphatase conjugated anti-rabbit IgG
as the secondary antibody.
different amounts of human IgA1. A serial dilution of RM124
was used as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.
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