Detection of human IgG2 in monkey serum. Sandwich ELISA using RM118 as the capture ELISA showing RM118 reacts only to human IgG2, A titer ELISA using RM118. The plate was ELISA showing RM118 does not react to monkey
Product Details
Product Name Anti-Human IgG2 Rabbit Monoclonal Antibody, Clone RM118
Product Description Rabbit monoclonal to Human IgG2
Catalog No. 31-1020-00
Clone Name RM118
Product Data Sheet Anti-Human IgG2 Rabbit Monoclonal Antibody Data Sheet RM118
Specificity RM118 reacts to the heavy chain of human IgG2. RM118 does not cross react to any other IgG subclasses (IgG1, IgG3, or IgG4), and shows no cross reactivity to IgM, IgA, IgD, or IgE. RM118 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Immunogen Human IgG2
Application ELISA
Species Reactivity Human
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
References for Human IgG2 antibody [RM118] Sulka KB et al. Lyme Disease-Associated IgG4 Autoantibodies Correlate with Synovial Pathology in Antibiotic-Refractory Lyme Arthritis. Arthritis Rheumatol. (2018) 10.1002/art.40566. IHC-P; Human. Read more (PubMed: 29790305)
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Product Images
Sandwich ELISA using RM118 as the capture
antibody, and biotinylated anti-human light
chains ( κ+ λ) antibody RM129 as the detection
antibody, followed by a HRP conjugated streptavidin.
antibody, and biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.
and not to any other IgG subclasses (IgG1, IgG3,
or IgG4), and no cross reactivity to IgM, IgA, IgD,
IgE, mouse IgG, rat IgG, or goat IgG.
coated with different amounts of human IgG2.
A serial dilution of RM118 was used as
the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary
antibody.
IgG. The plate was coated with Rhesus monkey IgG.
A serial dilution of RM118 and a monkey IgG binding
antibody (positive control) was used as the detection
antibody.
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