Sandwich ELISA using RM121 as the capture antibody Sandwich ELISA using RM121 as the capture antibody ELISA of human immunoglobulins shows RM121 A titer ELISA using RM121. The plate was coated
Product Details
Product Name Anti-Human IgM Rabbit Monoclonal Antibody, Clone RM120
Product Description Rabbit monoclonal to Human IgM
Catalog No. 31-1023-00
Clone Name RM121
Product Data Sheet Anti-Human IgM Rabbit Monoclonal Antibody Data Sheet RM121
Specificity RM121 reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE.
Immunogen Human IgM
Application ELISA
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
Species Reactivity Human
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
Immunocytochemistry (ICC): 0.5 µg/mL-2 µg/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Keywords
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Product Images
(100 ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.
reacts only to human IgM. No cross reactivity
with Human IgG, IgE, IgD, or IgA. The plate was
coated with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL of RM121 was used as
the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary
antibody.
with different amounts of human IgM. A serial dilution
of RM121 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.
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