RM167 specifically reacts to Histone H3 monomethy- Western Blot of recombinant histone H3.3 (1) and
Product Details
Product Name Anti-Monomethyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody, Clone RM167
Product Description Rabbit monoclonal to Monomethylated Histone H3 Lysine 18, H3K18me1, Histone H3 (monomethyl K18)
Catalog No. 31-1076-00
Clone Name RM167
Product Data Sheet Anti-Monomethyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody Data Sheet RM167
Specificity RM167 reacts to Histone H3 monomethylated at Lysine 18 (K18me1). No cross reactivity with nonmodified Lysine 18 or dimethylated Lysine 18 (K18me2), or other methylations in histone H3.
Immunogen A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys18).
Application Western Blot (WB)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 1 µg/mL – 2 µg/mL;
ELISA: 0.2 µg/mL - 1 µg/mL;
Multiplex: 0.1 µg/mL – 0.5 µg/mL.
Keywords
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Product Images
lated at Lysine 18 (K18me1). No cross reactivity with
nonmodified Lysine 18 (K18Ctrl) or dimethylated Lysine
18 (K18me2), or other methylations in histone H3.
acid extracts of HeLa cells (2), using RM167 at 1
µg/mL, showed a band of histone H3 monomethy-
lated at Lysine 18 (K18me1) in HeLa cells.
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