RM140 specifically reacts to Histone H3 monomethylated at Western Blot of recombinant histone H3.3 (1) and acid extracts ChIP performed on HeLa cells using H3K4me1 antibody Immunocytochemistry of HeLa cells treated with sodium butyrate,
Product Details
Product Name Anti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM140
Product Description Rabbit monoclonal to Monomethylated Histone H3 Lysine 4, H3K4me1, Histone H3 (monomethyl K4)
Catalog No. 31-1046-00
Clone Name RM140
Product Data Sheet Anti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM140
Specificity RM140 reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3
Immunogen A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys4)
Application Western Blot (WB)
Chromatin IP (ChIP)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.2 µg/mL – 1 µg/mL;
ChIP: 2 µg/mL- 10 µg/mL;
ICC: 1 µg/mL - 2 µg/mL;
ELISA: 0.2 µg/mL - 1 µg/mL;
Multiplex: 0.1 µg/mL – 0.5 µg/mL.
Keywords
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References Shah RN et al Examining the Roles of H3K4 Methylation States with Systematically Characterized Antibodies. Mol Cell. (2018), 10.1016/j.molcel.2018.08.015. ICeChIP. Read more (PubMed: 30244833)
Product Images
Lysine 4 (K4me1). No cross reactivity with dimethylated
Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or
other methylations in histone H3.
of HeLa cells (2), using RM140 at 0.5 µg/mL, showed a band of
histone H3 monomethylated at Lysine 4 (K4me1) in HeLa cells.
(RM140, 5 µg). Real-time PCR was performed using primers
specific to the gene indicated.
using Monomethyl-Histone H3 (Lys4) Rabbit mAb RM140 (red).
Actin filaments have been labeled with fluorescein phalloidin (green).
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