RM180 specifically reacts to Histone H3 monomethy- Western Blot of acid extracts of HeLa cells (1) and
Product Details
Product Name Anti-Monomethyl-Histone H3 (Lys56) Rabbit Monoclonal Antibody, Clone RM180
Product Description Rabbit monoclonal to Monomethylated Histone H3 Lysine 56, H3K56me1, Histone H3 (monomethyl K56)
Catalog No. 31-1095-00
Clone Name RM180
Product Data Sheet Anti-Monomethyl-Histone H3 (Lys56) Rabbit Monoclonal Antibody Data Sheet RM180
Specificity RM180 reacts to Histone H3 monomethylated at Lysine 56 (K56me1). No cross reactivity with nonmodified Lysine 56 or other methylations in histone H3.
Immunogen A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys56).
Application Western Blot (WB)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 μg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 1 μg/mL – 2 μg/mL;
ELISA: 0.2 μg/mL - 1 μg/mL;
Multiplex: 0.1 μg/mL – 0.5 μg/mL.
Keywords
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Product Images
lated at Lysine 56 (K56me1). No cross reactivity with
nonmodified Lysine 56 (K56Ctrl), or other methylations
in histone H3.
recombinant histone H3.3 (2), using RM180 at 1 μg/mL,
showed a band of histone H3 monomethylated at
Lysine 56 (K56me1) in HeLa cells.
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