RM150 specifically reacts to Histone H3 monomethy- Western Blot of recombinant histone H3.3 (1) and ChIP performed on HeLa cells using H3K9me1 antibody Immunocytochemistry of HeLa cells treated with
Product Details
Product Name Anti-Monomethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM150
Product Description Rabbit monoclonal to Monomethylated Histone H3 Lysine 9, H3K9me1, Histone H3 (monomethyl K9)
Catalog No. 31-1064-00
Clone Name RM150
Product Data Sheet Anti-Monomethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody Data Sheet RM150
Specificity RM150 reacts to Histone H3 monomethylated at Lysine 9 (K9me1). No cross reactivity with non-modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.
Immunogen A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys9).
Application Western Blot (WB)
Chromatin IP (ChIP)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt.
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.2 µg/mL - 1 µg/mL;
ChIP: 2 µg/mL - 10 µg/mL
ICC: 0.5 µg/mL - 2 µg/mL;
ELISA: 0.2 µg/mL - 1 µg/mL;
Multiplex: 0.1 µg/mL – 0.5 µg/mL.
Keywords
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Product Images
lated at Lysine 9 (K9me1). No cross reactivity with non-
modified Lysine 9, dimethylated Lysine 9 (K9me2),
trimethylated Lysine 4 (K9me3), or other methylations
in histone H3.
acid extracts of HeLa cells (2), using RM150 at 0.5
µg/mL, showed a band of histone H3 monomethy-
lated at Lysine 9 (K9me1) in HeLa cells.
(RM150, 5µg). Real-time PCR was performed using
primers specific to the gene indicated.
sodium butyrate, using Monomethyl-Histone H3
(Lys9) Rabbit mAb RM150 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).
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