RM458 specifically reacts to Histone H4 monomethylated Western Blot of acid extracts of HeLa cells (2) and recombinant
Product Details
Product Name Anti-Monomethyl-Histone H4 (Lys12) Rabbit Monoclonal Antibody, Clone RM458
Product Description Rabbit monoclonal to Monomethyl-Histone H4 (Lys12)
Catalog No. 31-1350-00
Clone Name RM458
Product Data Sheet Anti-Monomethyl-Histone H4 (Lys12) Rabbit Monoclonal Antibody Data Sheet RM458
Specificity RM458 reacts to Histone H4 monomethylated at Lysine 12 (K12me1). No cross reactivity with non-modified Lysine 12, or other monomethylations in histone H4.
Immunogen A monomethyl-peptide corresponding to monomethyl-Histone H4 (Lys12).
Application Western Blot
ELISA
Luminex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage WB: 0.01 µg/mL – 0.5 µg/mL;
ELISA: 0.5 µg/mL - 1 µg/mL;
Luminex: 0.01 µg/mL – 2 µg/mL.
Keywords
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Product Images
at Lysine 12 (K12me1). No cross reactivity with non-modified
Lysine 12, or other monomethylations in histone H4.
histone H4 (1), using RM458 at 0.02 µg/mL, showed a band
of histone H4 monomethylated at Lysine 12 (K12me1) in HeLa cells.
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