RM457 specifically reacts to Histone H4 monomethylated Western Blot of acid extracts of HeLa cells (2) and
Product Details
Product Name Anti-Monomethyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody, Clone RM457
Product Description Rabbit monoclonal to Monomethyl-Histone H4 (Lys5)
Catalog No. 31-1349-00
Clone Name RM457
Product Data Sheet Anti-Monomethyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody Data Sheet RM457
Specificity RM457 reacts to Histone H4 monomethylated at Lysine 5 (K5me1). No cross reactivity with non-modified Lysine 5, or other monomethylations in histone H4.
Immunogen A monomethyl-peptide corresponding to monomethyl-Histone H4 (Lys5).
Application Western Blot
ELISA
Luminex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage WB: 0.1 µg/mL – 2 µg/mL;
ELISA: 0.5 µg/mL - 1 µg/mL;
Luminex: 0.2 µg/mL – 5 µg/mL.
Keywords
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Product Images
at Lysine 5 (K5me1). No cross reactivity with non-modified
Lysine 5, or other monomethylations in histone H4.
recombinant histone H4 (1), using RM457 at 0.2 µg/mL,
showed a band of histone H4 monomethylated at Lysine 5
(K5me1) in HeLa cells.
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