ELISA of mouse immunoglobulins shows RM218 A titer ELISA of mouse IgG3. The plate was coated
Product Details
Product Name Anti-Mouse IgG3 Rabbit Monoclonal Antibody, Clone RM218
Product Description Rabbit monoclonal to Mouse IgG3
Catalog No. 31-1094-00
Clone Name RM218
Product Data Sheet Anti-Mouse IgG3 Rabbit Monoclonal Antibody Data Sheet RM218
Specificity RM218 reacts to the Fab region of mouse IgG3. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG.
Immunogen Mouse IgG3
Application ELISA
Western Blot (WB)
Species Reactivity Mouse
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 μg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.005 μg/mL - 0.2 μg/mL;
Western Blot (WB): 0.1 μg/mL - 0.5 μg/mL.
Keywords
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Product Images
reacts to both mouse IgG3κ and IgG3λ; No cross
reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA,
IgE, human IgG, or rat IgG. The plate was coated
with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL, or 10 ng/mL of RM218 was
used as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.
with different amounts of mouse IgG3. A serial
dilution of RM218 was used as the primary antibody.
An alkaline phosphatase conjugated anti-rabbit IgG
as the secondary antibody.
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