Immunohistochemistry of mouse liver and ELISA of three different PEGylated BSAs using Western blot of BSA and PEGylated BSA (mPEG 5 kDa)
Product Details
Product Name Anti-PEG (methoxy group) Rabbit Monoclonal Antibody, Clone RM105
Description Rabbit monoclonal to Polyethylene Glycol
Catalog No. 31-1008-00
Clone Name RM105
Product Data Sheet Anti-mPEG Rabbit Monoclonal Antibody Data Sheet RM105
Specificity RM105 reacts to the methoxy group of Polyethylene glycol (PEG). It is specific for methoxypolyethylene glycol.
Immunogen KLH-PEG with terminal methoxy group
Application ELISA
Western Blot (WB)
Immunohistochemistry (IHC)
Species Reactivity All Species
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.01 µg/mL - 0.3 µg/mL;
Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL;
Western Blot (WB) : 0.05 µg/mL - 1 µg/mL.
Keywords
References for Anti-PEG rabbit monoclonal antibody [RM105] Lam SK et al. Endogenous arginase 2 as a potential biomarker for PEGylated arginase 1 treatment in xenograft models of squamous cell lung carcinoma. Oncogenesis. (2019), 10.1038/s41389-019-0128-0. WB. Read more (PubMed: 30808864)
Lam SK et al. Growth suppressive effect of pegylated arginase in malignant pleural mesothelioma xenografts. Respir Res. 10.1186/s12931-017-0564-3 (2017). WB, IF IHC. Read more (PubMed: 28464918)
Lam SK et al. Inhibition of ornithine decarboxylase 1 facilitates pegylated arginase treatment in lung adenocarcinoma xenograft models. Oncol Rep. 10.3892/or.2018.6598 (2018). WB, DotBlot Read more (PubMed: 30066894)
Xu Shi et al. Recombinant human arginase induces apoptosis through oxidative stress and cell cycle arrest in small cell lung cancer. Cancer Sci. 10.1111/cas.13782 (2018). WB, IF Read more (PubMed: 30155941)
McCallen J et al. Cross-Reactivity of Select PEG-Binding Antibodies to Other Polymers Containing a C-C-O Backbone. ACS Biomater. Sci. Eng. (2017), 10.1021/acsbiomaterials.7b00147. ELISA, Dot Blot; Human. Read more (PubMed: 7b00147)
Product Images
kidney using 0.5 µg/mL of RevMAb anti-PEG,
Clone RM105. The mouse was injected with
PEG-BSA or BSA for 3 hours before sampling.
anti-PEG RevMAb Clone RM105, followed by an
AP conjugated goat anti-rabbit IgG.
using 0.1 µg/mL of anti-PEG RevMAb Clone RM105.
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