Western Blot of lysates from 293 cells either, grown Immunohistochemical staining of formalin fixed and
Product Details
Product Name Anti-Phospho-Akt (Ser473) Rabbit Monoclonal Antibody, Clone RM251
Product Description Rabbit monoclonal to phospho-Akt (Ser473), AKT (phospho S473), AKT1/2/3 (phospho S473)
Catalog No. 31-1131-00
Clone Name RM251
Product Data Sheet Anti-Phospho-Akt (Ser473) Rabbit Monoclonal Antibody Data Sheet RM251
Specificity RM251 reacts to Akt only when phosphorylated at Ser473. There is no cross-reactivity with Akt without phosphorylation at Ser473. This antibody may also react to bovine, mouse or rat Phospho-Akt (Ser473), as predicted by immunogen homology.
Immunogen A phospho-peptide corresponding to human Phospho-Akt (Ser473)
Application Immunohistochemistry (IHC)
Western Blot (WB)
Species Reactivity Human
(Mouse, Rat, Bovine)
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 µL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Immunohistochemistry (IHC): 1:200-1:500 dilution;
Western Blot (WB): 1:1000-1:2000 dilution.
Keywords
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Product Images
in medium with serum, serum starved, or insulin
treated. Using Anti-Phospho-Akt (Ser473) RM251
at a 1:1000 dilution or anti-Akt1 RM252 at a 1:1000
dilution.
paraffin embedded human breast cancer tissue
sections using Anti-Phospho-Akt (Ser473) RM251
at a 1:400 dilution.
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