Western Blot of acid extracts of HeLa cells treated Immunocytochemistry of HeLa cells using Anti-Phospho-
Product Name Anti-Phospho-Histone H2B (Ser14) Rabbit Monoclonal Antibody [RM238]
Product Description Rabbit monoclonal to Phospho-Histone H2B (Ser14), H2BS14p, Histone H2B (S14 phosphorylation)
Catalog No. 31-1115-00
Clone Name RM238
Product Data Sheet Anti-Phospho-Histone H2B (Ser14) Rabbit Monoclonal Antibody Data Sheet RM238
Specificity RM238 reacts to Histone H2B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones.
Immunogen A phospho-peptide corresponding to Phospho-Histone H2B (Ser14).
Application Western Blot (WB)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL - 2 ug/mL;
ICC: 1 μg/mL - 2 μg/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 1 ug/mL.
Keywords . Product Images
or non-treated with Nocodazole. Using RM238
at 0.5 ug/mL, showed a band of Histone H2B
phosphorylated at Serine 14 in HeLa cells.
Histone H2B (Ser14) Rabbit mAb RM238 (red) and
DAPI (blue). Actin filaments have been labeled with
fluorescein phalloidin (green).
Western Blot of acid extracts of HeLa cells treated
Immunocytochemistry of HeLa cells using Anti-Phospho-