Western Blot of acid extracts of HeLa cells treated Immunocytochemistry of HeLa cells using Anti-Phospho-
Product Details
Product Name Anti-Phospho-Histone H2B (Ser14) Rabbit Monoclonal Antibody, Clone RM238
Product Description Rabbit monoclonal to Phospho-Histone H2B (Ser14), H2BS14p, Histone H2B (S14 phosphorylation)
Catalog No. 31-1115-00
Clone Name RM238
Product Data Sheet Anti-Phospho-Histone H2B (Ser14) Rabbit Monoclonal Antibody Data Sheet RM238
Specificity RM238 reacts to Histone H2B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones.
Immunogen A phospho-peptide corresponding to Phospho-Histone H2B (Ser14).
Application Western Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 µg/mL - 2 µg/mL;
ICC: 1 μg/mL - 2 μg/mL;
ELISA: 0.2 µg/mL - 1 µg/mL;
Multiplex: 0.1 µg/mL - 1 µg/mL.
Keywords
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Product Images
or non-treated with Nocodazole. Using RM238
at 0.5 µg/mL, showed a band of Histone H2B
phosphorylated at Serine 14 in HeLa cells.
Histone H2B (Ser14) Rabbit mAb RM238 (red) and
DAPI (blue). Actin filaments have been labeled with
fluorescein phalloidin (green).
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