Western Blot of acid extracts of HeLa cells treated Immunocytochemistry of HeLa cells using Phospho-Histone H3
Product Details
Product Name Anti-Phospho-Histone H3 (Ser10) Rabbit Monoclonal Antibody, Clone RM163
Product Description Rabbit monoclonal to Phospho-Histone H3 (Ser10), Histone H3 (S10 phosphorylation)
Catalog No. 31-1070-00
Clone Name RM163
Product Data Sheet Anti-Phospho-Histone H3 (pSer10) Rabbit Monoclonal Antibody Data Sheet RM163
Specificity RM163 reacts to Histone H3 phosphorylated at Serine 10. No cross reactivity with other phosphorylated histones.
Immunogen A phospho-peptide corresponding to Phospho-Histone H3 (Ser10).
Application Western Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 µg/mL - 2 µg/mL;
ICC: 0.5 µg/mL - 2 µg/mL;
ELISA: 0.2 µg/mL - 1 µg/mL;
Multiplex: 0.1 µg/mL – 1 µg/mL.
Keywords
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Product Images
or non-treated with Nocodazole. Using RM163 at
0.5 µg/mL, showed a band of Histone H3 phospho-
rylated at serine 10 in HeLa cells.
(Ser10) Rabbit mAb RM163 (red). Actin filaments have been
labeled with fluorescein phalloidin (green).
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