Western Blot of acid extracts of HeLa cells treated
Product Details
Product Name Anti-Phospho-Histone H3 (Thr11) Rabbit Monoclonal Antibody, Clone RM164
Product Description Rabbit monoclonal to Phospho-Histone H3 (Thr11), Histone H3 (Thr11 phosphorylation)
Catalog No. 31-1073-00
Clone Name RM164
Product Data Sheet Anti-Phospho-Histone H3 (Thr11) Rabbit Monoclonal Antibody Data Sheet RM164
Specificity RM164 reacts to Histone H3 phosphorylated at Threonine 11. No cross reactivity with other phosphorylated histones.
Immunogen A phospho-peptide corresponding to Phospho-Histone H3 (Thr11).
Application Western Blot (WB)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 μg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 μg/mL - 2 μg/mL;
ELISA: 0.2 μg/mL - 1 μg/mL;
Multiplex: 0.1 μg/mL – 1 μg/mL.
Keywords
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Product Images
or non-treated with Nocodazole. Using RM164 at
0.5 μg/mL, showed a band of Histone H3 phospho-
rylated at threonine 11 in HeLa cells.
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