Western Blot of acid extracts of HeLa cells non-treated Immunocytochemistry of HeLa cells using Phospho-Histone Immunocytochemistry of HeLa cells using Phospho-Histone Immunohistochemical staining of formalin fixed and
Product Details
Product Name Anti-Phospho-Histone H3 (Thr3) Rabbit Monoclonal Antibody, Clone RM159
Product Description Rabbit monoclonal to Phospho-Histone H3 (Thr3), Histone H3 (Thr3 phosphorylation)
Catalog No. 31-1072-00
Clone Name RM159
Product Data Sheet Anti-Phospho-Histone H3 (Thr3) Rabbit Monoclonal Antibody Data Sheet RM159
Specificity RM159 reacts to Histone H3 phosphorylated at Threonine 3. No cross reactivity with other phosphorylated histones
Immunogen A phospho-peptide corresponding to Phospho-Histone H3 (Thr3)
Application Western Blot (WB)
Immunohistochemistry (IHC)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.1 µg/mL - 1 µg/mL;
IHC: 0.5 µg/mL - 2 ug/mL;
ICC: 0.5 µg/mL - 2 µg/mL;
ELISA: 0.2 µg/mL - 1 µg/mL;
Multiplex: 0.1 µg/mL – 1 µg/mL.
Keywords
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Product Images
(1) or treated (2) with Nocodazole, and recombinant
Histone H3.3 (3). Using RM159 at 0.1 µg/mL, showed
a band of Histone H3 phosphorylated at threonine 3
in HeLa cells
.
H3 (Thr3) Rabbit mAb RM159 (red). Actin filaments have been
labeled with fluorescein phalloidin (green).
H3 (Thr3) Rabbit mAb RM159 (red). Actin filaments have been
labeled with fluorescein phalloidin (green).
paraffin embedded human breast cancer tissue section
using anti-Phospho-Histone H3 (Thr3) rabbit monoclonal
antibody, Clone RM159, at a 1 µg/mL.
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